TY - JOUR ID - 15949 TI - Topical green tea formulation with anti-hemorrhagic and antibacterial effects JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Kalalinia, Fatemeh AU - Amiri, Nafise AU - Mehrvarzian, Niloufar AU - Fazly Bazzaz, Bibi Sedigheh AU - Iranshahi, Mehrdad AU - Shahroodi, Azadeh AU - Arabzadeh, Sepideh AU - Abbaspour, Mohammadreza AU - Badiee Aaval, Shapour AU - Movaffagh, Jebrail AD - Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran AD - Complementary Medicine Research Center, Faculty of Traditional Medicine, Mashhad University of Medical Sciences, Mashhad, lran Y1 - 2020 PY - 2020 VL - 23 IS - 8 SP - 1085 EP - 1090 KW - Bleeding KW - green tea KW - hemostasis KW - Polyvinyl alcohol KW - Tannin KW - Topical formulation DO - 10.22038/ijbms.2020.41397.9782 N2 - Objective(s): Potentially preventable death from uncontrolled hemorrhage clearly indicates the importance of simple, fast and efficient ways to achieving hemostasis. The aim of this study was to develop a topical formulation of green tea extract for reducing bleeding that can be helpful in hemorrhage control.Materials and Methods: Hydroalcoholic extract of green tea was isolated from Camellia sinensis and formulated in polyvinyl alcohol (PVA) to achieve two concentrations of 2% and 4% v/v. Folin-Ciocalteau assay was used to determine the total amount of tannins in extract. Rheological behavior of solutions was investigated by measuring viscosity at shear rates of 0–200 sec−1. Quantitative and qualitative microbial limit tests and minimum inhibitory concentration (MIC) assay were done. The effect of formulations on bleeding time was evaluated in an animal model. Results: The total amount of tannin in green tea extract was 3.8% w/w and addition of green tea significantly increased the viscosity of PVA. The results of MIC assay showed that PVA could not inhibit the growth of bacteria, while, 716 µg/ml of green tea and 2860 µg/ml of green tea/PVA 4% inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa. In an animal study both 2% and 4% formulations were able to stop hemorrhage approximately at an equal time compared with tranexamic acid (TXA) 50 mg/ml as a control and the lowest bleeding time was 6.4±0.51 sec for green tea/PVA 4%.Conclusion: Based on our results, the topical formulation of green tea extract in PVA has a great potential for anti-hemorrhage applications. UR - https://ijbms.mums.ac.ir/article_15949.html L1 - https://ijbms.mums.ac.ir/article_15949_d29d9674f7d64707ee519ef34cd0849b.pdf ER -