TY - JOUR ID - 1665 TI - Sphingomyelin Liposomes Containing Soluble Leishmania major antigens Induced Strong Th2 Immune Response in BALB/c Mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Chavoshian, Omid AU - Biari, Nazanin AU - Badiee, Ali AU - Khamesipour, Ali AU - Abbasi, Azam AU - Saberi, Zahra AU - Jalali, Seyed Amir AU - Jaafari, Mahmoud Reza AD - Nanotechnology Research Center, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran AD - Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran AD - Nanotechnology Research Center, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran 2 Biotechnology Research Center, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran Y1 - 2013 PY - 2013 VL - 16 IS - 9 SP - 965 EP - 972 KW - Leishmaniasis Liposome Vaccine DO - 10.22038/ijbms.2013.1665 N2 -   Objective(s): Soluble Leishmania antigens (SLA) provide suitable protection against leishmaniasis in murine model when delivered by an appropriate delivery system. Liposomes have been shown to be suitable vaccine delivery systems against leishmaniasis, however, the phospholipase-A (PLA) activity of SLA is a drawback to prepare a stable liposomal SLA. One strategy to overcome this problem might be using a lipid which is resistant to PLA activity of SLA such as sphingomyelin (SM). The aim of this study was to evaluate the effect of stable SM liposomes containing SLA on the immune response induced against leishmaniasis in BALB/c mice .   Materials and Methods: BALB/c mice were immunized subcutaneously, three times with 2-week intervals, with SLA, SM-liposome-SLA, empty liposome or buffer. As criteria for protection, footpads swelling at the site of challenge and foot parasite loads were assessed. The immune responses were also evaluated by determination of IgG subtypes and the level of IFN-γ and IL-4 in cultured splenocytes. Results: The group of mice receiving SM-liposome-SLA, showed a significant large footpad swelling, higher parasite burden in foot and higher IL-4 level compared to the group immunized with buffer. In terms of IgG and IgG isotypes, there was no significant difference between the mice receiving SM-liposome-SLA and the mice that received buffer. Moreover, the immune response induced by SM-liposome-SLA showed no significant difference compared with the one caused by SLA alone. Conclusion: It is concluded that SM-liposome-SLA is not an appropriate strategy to induce Th1 immune response and protect the mice against Leishmaniasis; however, SM-liposomes could be suitable vaccine delivery systems when a Th2 response is needed. UR - https://ijbms.mums.ac.ir/article_1665.html L1 - https://ijbms.mums.ac.ir/article_1665_17a9278f5d2dea04495d6b5f98036dc7.pdf ER -