TY - JOUR ID - 16779 TI - Celecoxib inhibits acute edema and inflammatory biomarkers through peroxisome proliferator-activated receptor-γ in rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Houshmand, Gholamreza AU - Naghizadeh, Bahareh AU - Ghorbanzadeh, Behnam AU - Ghafouri, Zahra AU - Goudarzi, Mehdi AU - Mansouri, Mohammad taghi AD - Department of Pharmacology, School of Medicine, Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran AD - Department of Anesthesiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA AD - Department of Pharmacology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran AD - Department of Biochemistry Biophysics and Genetics, School of Medicine, Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran AD - Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Y1 - 2020 PY - 2020 VL - 23 IS - 12 SP - 1544 EP - 1550 KW - Carrageenan Celecoxib Cytokines Oxidative stress Pioglitazone PPAR KW - γ Rat DO - 10.22038/ijbms.2020.43995.10315 N2 - Objective(s): Celecoxib (CLX), a selective cyclooxygenase-II (COX-2) inhibitor, has been used for management of several inflammatory disorders. The present study aimed to explore the role of peroxisome proliferator-activated receptor-gamma (PPARγ) in CLX induced anti-inflammatory response in rats.Materials and Methods: Carrageenan-induced paw edema was used as an acute inflammation model. Rats were treated with various intra-peritoneal (IP) doses of CLX (0.3–30 mg/kg) and pioglitazone (PGL; PPARγ agonist, 1–20 mg/kg) alone or in combination. Amounts of PPARγ, COX-2, and prostaglandin E2 (PGE2) in paw tissue, and extents of TNF-α and IL-10 in serum were measured. Moreover, levels of oxidative stress parameters as malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx) activity in the cortex, hippocampus, and paw tissues were also determined.  Results: CLX and PGL dose-dependent administration (IP), alone or in combination reduced carrageenan-induced paw edema. Further, both agents, alone or in combination, reduced either the amounts of COX-2, PGE2, and MDA in the inflamed paw, and the levels of TNF-α in serum which were elevated by carrageenan. Both drugs also increased both levels of PPARγ, GSH, GPx activity in paws, and serum levels of IL-10 that were decreased by carrageenan. Intraplantar injection of GW-9662 (IPL), a selective PPARγ antagonist, inhibited all biochemical modifications caused by both single and combined drug treatments. Conclusion: CLX produced its anti-inflammatory effects probably through PPARγ receptor activation. Besides, increased anti-inflammatory effects of CLX with PGL suggest that their combination might be applied for the clinical management of inflammation especially in patients suffering from diabetes. UR - https://ijbms.mums.ac.ir/article_16779.html L1 - https://ijbms.mums.ac.ir/article_16779_aac7a9997f295d8d27ab3458b43ea17b.pdf ER -