TY - JOUR ID - 17462 TI - c-kit+ cells offer hopes in ameliorating asthmatic pathologies via regulation of miRNA-133 and -126 JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Rahbarghazi, Reza AU - Kihanmanesh, Rana AU - Rezaie, Jafar AU - Mirershadi, Fatemeh AU - Heiran, Hossain AU - Saghaei Bagheri, Hesam AU - Saberianpour, Shirin AU - Rezabakhsh, Aysa AU - Delkhosh, Aref AU - Bagheri, Yasin AU - Rajabi, Hadi AU - Ahmadi, Mahdi AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Solid Tumor Research Center, Research Institute for Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Department of Physiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran AD - Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Science, Mashhad, Iran AD - Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran AD - Young Researchers and Elite Club, Tabriz Branch Islamic Azad university, Tabriz, Iran Y1 - 2021 PY - 2021 VL - 24 IS - 3 SP - 369 EP - 376 KW - Cell therapy KW - Histological changes KW - Lung KW - Ovalbumin KW - Rat DO - 10.22038/ijbms.2021.49008.11231 N2 - Objective(s): There are still challenges regarding c-kit+ cells’ therapeutic outcome in the clinical setting. Here, we examined the c-kit+ cell effect on the alleviation of asthma by modulating miRNAs expression.Materials and Methods: To induce asthma, male rats were exposed to ovalbumin. Bone marrow-derived c-kit+ cells were enriched by MACS. Animals were classified into four groups (6 rats each). Control rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally containing 3×105 c-kit+ and c-kit- cells. Cells were stained with Dil fluorescent dye to track in vivo condition. Pathological changes were monitored in asthmatic rats after transplantation of c-kit+ and c-kit- cells. Serum levels of IL-4 and INF-γ were measured by ELISA. Transcription of miRNAs (-126 and 133) was assessed by real-time PCR analysis.Results: Pathological examination and Th1 and Th2 associated cytokine fluctuation confirmed the occurrence of asthma in rats indicated by chronic changes and prominent inflammation compared with the control group (p <0.05). Both c-kit+ and c-kit- cells were verified in pulmonary niche. Administration of c-kit positive cells had the potential to change INF-γ/IL-4 ratio close to the normal values compared with matched-control asthmatic rats (p <0.05). We also found that c-kit+ cells regulated the expression of miRNA-126 and -133, indicated by an increase of miRNA-133 and decrease of miRNA-126 compared with cell-free sensitized groups (p <0.05). Conclusion: c-kit- cells were unable to promote any therapeutic outcomes in the asthmatic milieu. c-kit+ cells had the potential to diminish asthma-related pathologies presumably by controlling the transcription of miRNA-126 and -133. UR - https://ijbms.mums.ac.ir/article_17462.html L1 - https://ijbms.mums.ac.ir/article_17462_81e697fef497e998e46655954d97af6d.pdf ER -