TY - JOUR ID - 17610 TI - Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Nazir, Humera AU - Aziz, Mubashar AU - Mirani, Zulfiqar Ali AU - Sheikh, Ahsan Sattar AU - Saeed, Muhammad Qamar AU - Hussain, Masroor AU - Khan, Aleem Ahmed AU - Ruby, Tahira AU - Rauf, Naseem AD - Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan AD - Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan AD - Department of Microbiology, PCSIR Laboratories Complex, Karachi 74000, Pakistan AD - Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan AD - Department of Biotechnology, University of Science & Technology Bannu, Pakistan. AD - Department of Zoology, The Islamia University of Bbahawalpur,61300, Bahawalpur, Pakistan AD - PCSIR Laboratories Islamabad, Pakistan Y1 - 2021 PY - 2021 VL - 24 IS - 3 SP - 400 EP - 407 KW - Escherichia coli KW - ESBL KW - MDR KW - PCR KW - UTI DO - 10.22038/ijbms.2021.47095.10865 N2 - Objective(s): Emergence of multidrug resistance has reduced the choice of antimicrobial regimens for UTIs. To understand the association of phenotype and genotype among uropathogens.Materials and Methods: Six hundred and twenty-eight (628) urine samples were collected and analyzed. Antibiotic sensitivity pattern was determined by the Kirby-Bauer Disc Diffusion Method and minimum inhibitory concentration (MIC) was tested by the E test. Fluoroquinolone resistant mutations in QRDR of gyrA and ParC, phylogenetic groups, and PAIusp subtype were detected by PCR.Results: Most prevalent uropathogens were Escherichia coli (53.2%) followed by Klebsiella pneumoniae (21%). Multidrug- resistance was observed in > 50% cases for third-generation cephalosporins and ciprofloxacin and lowest in meropenem. E. coli (66.2%) and K. pneumonia (64.4%) were extended-spectrum β-lactamases (ESBLs) producers. MIC to trimethoprim-sulfamethoxazole was highest in E. coli (>1024 µg/ml). In 80 (24%) of the 334 E. coli isolates analyzed in detail, 54 fluoroquinolones (FQ) resistant isolates carried mutations (S83L, D87N, S80I, E84V) in QRDR of gyrA and ParC. Out of 54 FQ-resistant isolates, 43 (79.6%) isolates belonged to the phylogenetic group B2, and 11(20.4%) belonged to group D. Isolates belonged to group B2, 38 (88.4%) of the 43 isolates carried PAIusp subtype IIa and high frequency of mutation E84V in ParC was detected in 37 (97.4%). Other mutations, such as S80I, S83L in gyrA and D87N in ParC were found in all resistant isolates.Conclusion: Correlations between phenotype and genotype provided a basis to understand the resistance development in uropathogens, and PAIusp subtyping indicated that E. coli belonged to the B2 group. UR - https://ijbms.mums.ac.ir/article_17610.html L1 - https://ijbms.mums.ac.ir/article_17610_a8c02d7ed47723482ea5e0a4ca8953fc.pdf ER -