TY - JOUR ID - 17755 TI - Voltage-gated potassium channels are involved in oxymatrine-regulated islet function in rat islet β cells and INS-1 cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Gao, Jingying AU - xia, Lixia AU - Wei, Yuanyuan AD - Department of Pediatrics, Shanxi Medical University, Taiyuan, China Y1 - 2021 PY - 2021 VL - 24 IS - 4 SP - 460 EP - 468 KW - Apoptosis Diabetes mellitus Insulin secretion Oxymatrine Potassium channel Voltage KW - gated DO - 10.22038/ijbms.2021.52449.11850 N2 - Objective(s): Oxymatrine can regulate glucose metabolism. But the underlying mechanisms remain unclear. We investigated the relationship of oxymatrine and voltage-gated potassium (Kv) channel in rat islet β cells and INS-1 cells.Materials and Methods: Insulin secretion and Kv channel currents were tested by radioimmunoassay and patch-clamp technique, respectively. The INS-1 cell viability was detected using cell counting kit-8 experiments. Flowcytometry analysis and western blot were employed for cell apoptosis and protein levels, respectively. INS-1 cell proliferation was assessed by the 5-Ethynyl-2’- deoxyuridine method. Results: Oxymatrine potentiated insulin secretion at high glucose (p Conclusion: The results indicate that oxymatrine can stimulate insulin secretion and decrease kv channel currents in islet β cells. Besides, oxymatrine also increases cell viability, proliferation, and reduces cell apoptosis in INS-1 cells. The effects of oxymatrine are related to kv channels. This finding provides new insight into the mechanisms of oxymatrine-regulated islet function. UR - https://ijbms.mums.ac.ir/article_17755.html L1 - https://ijbms.mums.ac.ir/article_17755_742e709253b90b76eb5cae310ff59e81.pdf ER -