TY - JOUR ID - 18389 TI - Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - GULER, Mustafa Can AU - Tanyeli, Ayhan AU - GUZEL ERDOGAN, Derya AU - Eraslan, Ersen AU - Comakli, Selim AU - Polat, Elif AU - DOGANAY, Songul AD - Department of Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey AD - Department of Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey AD - Department of Physiology, Yozgat Bozok University, Faculty of Medicine, Yozgat, Turkey AD - Department of Pathology, Atatürk University, Veterinary Faculty, Erzurum, Turkey AD - Department of Nutrition and Dietetics, Faculty of Health Sciences, Erzurum Technical University, Erzurum, Turkey Y1 - 2021 PY - 2021 VL - 24 IS - 7 SP - 935 EP - 942 KW - Autophagy KW - Caspase-3 KW - Detorsion KW - NF-κB KW - Ovarian KW - Torsion KW - Urapidil DO - 10.22038/ijbms.2021.57196.12736 N2 - Objective(s): This study aimed to determine anti-inflammatory, antioxidant, and antiapoptotic properties of urapidil (Ura) against ovarian torsion detorsion (T/D) injury in rats. Materials and Methods: 40 female Wistar albino rats were grouped as sham, T/D, T/D+dimethyl sulfoxide (DMSO), T/D+Urapidil (Ura) 0.5 mg/kg (low dose), and T/D+Urapidil (Ura) 5 mg/kg (high dose) groups. In treatment groups, Ura was administered intraperitoneally just before detorsion. Biochemical parameters (TAS, TOS, MDA, MPO, and SOD) and immunohistochemical (IL-1β, TNF-α, NF-κB, LC3B, and Caspase-3) analyzes were performed.Results: In the T/D group, OSI and MPO levels were elevated significantly while TAS values decreased compared with the sham group. A significant difference occurred in the low dose treatment group in TAS and OSI levels compared with the T/D group. In the high dose treatment group, significant elevation in TAS but  reduction in OSI and MDA levels were observed compared with the T/D group. Immunohistochemical staining resulted in IL-1β, TNF-α, NF-κB, LC3B, and caspase-3 immunopositivity in the T/D group, while Ura treatment decreased those parameters. Intensive congestion and hemorrhage were observed in the T/D group, but contrary to this, treatment groups had alleviated congestion and hemorrhage.Conclusion: These results suggest that Ura demonstrated protective effects against ovarian T/D injury via anti-oxidative, anti-inflammatory, and anti-apoptotic features.  UR - https://ijbms.mums.ac.ir/article_18389.html L1 - https://ijbms.mums.ac.ir/article_18389_4d764afeb6ddb2e7ba8f18d481fb4037.pdf ER -