TY - JOUR ID - 18586 TI - Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Jiang, Xi AU - Hu, Ying AU - Zhou, Yingjie AU - Chen, Jin AU - Sun, Chonglu AU - Chen, Ziwei AU - Jing, Changfeng AU - Xu, Lexing AU - Liu, Fuhe AU - Ni, Wenjuan AU - Yu, Xuefeng AU - Chen, Lei AD - Zhejiang University Mingzhou Hospital, Ningbo, 315000, China AD - Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China Y1 - 2021 PY - 2021 VL - 24 IS - 9 SP - 1247 EP - 1253 KW - AMP-activated protein kinases KW - Endometritis KW - Inflammation KW - Irisin KW - Lipopolysaccharide NF-κB DO - 10.22038/ijbms.2021.56781.12678 N2 - Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation.  Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis. UR - https://ijbms.mums.ac.ir/article_18586.html L1 - https://ijbms.mums.ac.ir/article_18586_e53c599fb102d95022abf185c4628743.pdf ER -