TY - JOUR ID - 18758 TI - Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Azadpour, Mojgan AU - Farajollahi, Mohammad Morad AU - Dariushnejad, Hassan AU - Varzi, Ali Mohammad AU - Varezardi, Amir AU - Barati, Mitra AD - Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran AD - Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences,Tehran, Iran AD - Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran AD - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Y1 - 2021 PY - 2021 VL - 24 IS - 10 SP - 1446 EP - 1454 KW - Cytokines KW - Nanoparticles KW - PLGA compound KW - Peritoneal macrophages KW - Silymarin DO - 10.22038/ijbms.2021.59312.13161 N2 - Objective(s): Silymarin (SM) is a natural antioxidant compound with good anti-inflammatory effects, but its poor water solubility restricts its usage. Today, nanomaterial compounds (such as PLGA Poly D, L-lactic-co-glycolic acid) can provide a proper drug delivery system and help improve the accessibility of bioactive compounds to cells and tissues. Materials and Methods: In this study, PLGA nanoparticles (NPs) containing SM (SM-PLGA) were synthesized and characterized and their biological effects were evaluated on M2 macrophage polarization to regulate inflammation. SM-PLGA NPs were fabricated by the oil in water emulsion (O/W) method. Macrophages (MQs) were isolated from mouse peritoneum by the cold RPMI lavage protocol. Primary mouse MQ cells were treated by SM and SM-PLGA NPs and then stimulated with lipopolysaccharide (LPS). M2 polarization was evaluated by measurements of cytokine secretion levels (TNF-α, IL1-β, and IL-10), flow cytometry markers (F4/80, CD11b, CD38, and CD206), and the expression of specific proteins (M2 Ym1 and Fizz1).Results: SM-PLGA characterization showed that NPs were fabricated in the desired form. SM and SM-PLGA decreased pro-inflammatory cytokines (TNF-α and IL1-β) and increased IL10 as an anti-inflammatory cytokine. On the other hand, the M2-associated markers and proteins increased following treatment with SM and SM-PLGA. Post-hoc analysis indicated that these changes were more pronounced in the SM-PLGA group.Conclusion: This study revealed that SM-PLGA could markedly promote M2 polarization, thereby providing a valuable medical approach against sepsis and septic shock. UR - https://ijbms.mums.ac.ir/article_18758.html L1 - https://ijbms.mums.ac.ir/article_18758_5474a061ba97022bfe0b46420fbd8902.pdf ER -