TY - JOUR ID - 19301 TI - MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Chen, Xiao-Guang AU - Dou, Bing-Hua AU - An, Jin-Dou AU - Feng, Song AU - Liu, Na AU - Sheng, Guang-Yao AD - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. China Y1 - 2022 PY - 2022 VL - 25 IS - 1 SP - 46 EP - 52 KW - ALL KW - Apoptosis KW - FOXN3 KW - Glycolysis KW - Growth KW - MAGI2-AS3 KW - miR-452-5p DO - 10.22038/ijbms.2021.58963.13095 N2 - Objective(s): MAGI2-AS3 is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI2-AS3 in ALL.Materials and Methods: qPCR and Western blotting were adopted for detection of target molecular expression. Growth and apoptosis were determined by CCK8 assay and Annexin V/PI staining. Glycolysis was detected by commercial kits. The direct binding between miR-452-5p and MAGI2-AS3 or FOXN3 was assessed by luciferase reporter assay. Tumor growth was measured in nude mice in vivo.Results: MAGI2-AS3 was down-regulated in ALL. Enforced expression of MAGI2-AS3 inhibited growth and glycolysis while promoting apoptosis of ALL cells. Moreover, MAGI2-AS3 up-regulated FOXN3 via sponging miR-452-5p. FOXN3 depletion abrogated MAGI2-AS3-mediated anti-cancer action. More importantly, MAGI2-AS3 repressed ALL cell growth in nude mice through regulation of miR-452-5p/FOXN3. Conclusion: MAGI2-AS3 inhibits ALL development via modulating miR-452-5p/FOXN3. UR - https://ijbms.mums.ac.ir/article_19301.html L1 - https://ijbms.mums.ac.ir/article_19301_2880897d75bd5e6d764067c82942d63b.pdf ER -