TY - JOUR ID - 20781 TI - Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Hadavi, Mirhossein AU - H. Najdegerami, Ebrahim AU - Nikoo, Mehdi AU - Nejati, Vahid AD - Department of Biology, Faculty of Science, Urmia University, Urmia, Iran AD - Department of Pathobiology and Quality Control, Artemia & Aquaculture Research institute, Urmia University, Urmia, Iran Y1 - 2022 PY - 2022 VL - 25 IS - 8 SP - 954 EP - 963 KW - Autophagy KW - Bioactive peptides KW - Fatty liver KW - Insulin KW - Oxidative Enzymes KW - Whiteleg shrimp DO - 10.22038/ijbms.2022.62167.13761 N2 - Objective(s): The effects of protein hydrolysates (FP) from Litopenaeus vannamei on oxidative stress, and autophagy gene expression was investigated in the NAFLD-induced rats.  Materials and Methods: For this purpose, twenty-four male rats were divided into four groups: Control, High-fat diet (HFD), FP20+HFD, and FP300+HFD (20 and 300 mg FP /kg rat body weight) and fed for 70 days. Results: The results indicated that the rat body and relative weight of the liver were not affected by experimental treatments (P>0.05) although the highest relative weight of the liver was observed in HFD treatment. The highest and lowest values for antioxidant enzymes and MDA concentration were observed in FP treatments (P<0.05). Also, the results showed that FP significantly decreased liver enzymes (ALT, AST) in the liver in comparison with HFD treatment (P<0.05). Plasma biochemical indices were investigated and the lowest amylase, ALP, fasting glucose, insulin, HOMA-IR, triglycerides, cholesterol, and inflammation cytokines (TNF-α, IL-6) were seen in the FP treatments which had a significant difference with HFD (P<0.05). Autophagy gene expression in the liver cells was affected by experimental diets and the lowest expression of Beclin-1 and Atg7 was observed in HFD and FP300 treatments. Interestingly, the highest expression of LC3-ɪ and P62 was seen in HFD and FP treatments, not in the control. Conclusion: Overall, the results of this experiment indicated that FPs extracted from Whiteleg shrimp at 50 °C improve the oxidative status, glucose metabolism, and autophagy gene expression and could be used as a useful nutritional strategy in fatty liver prevention. UR - https://ijbms.mums.ac.ir/article_20781.html L1 - https://ijbms.mums.ac.ir/article_20781_382f0bbf77caa56a3d855e12d9ee1d36.pdf ER -