TY - JOUR ID - 21092 TI - MitoTEMPOL modulates mitophagy and histopathology of Wistar rat liver after streptozotocin injection JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Virgana, Rova AU - Gunadi, Julia Windi AU - Atik, Nur AU - Limdawati, Kwee AU - Jasaputra, Diana Krisanti AU - Wahyudianingsih, Roro AU - Suardi, Nadya Naurah Adrina AU - Soetadji, Ray Sebastian AU - Goenawan, Hanna AU - Lesmana, Ronny AU - Kartasasmita, Arief Sjamsulaksan AD - Department of Ophthalmology, Universitas Padjadjaran, Bandung, West Java, Indonesia AD - Department of Physiology, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia AD - Biology Cell Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia AD - Department of Internal Medicine, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia AD - Department of Pharmacology, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia AD - Department of Pathology Anatomy, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia AD - Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia AD - Physiology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia Y1 - 2022 PY - 2022 VL - 25 IS - 11 SP - 1382 EP - 1388 KW - Anti-Oxidants KW - Lipid droplet KW - Mitochondrial dynamics KW - Mitophagy KW - Metabolic zone KW - Oxidative stress DO - 10.22038/ijbms.2022.65285.14375 N2 - Objective(s): This study aims to explore the effect of mitoTEMPOL on histopathology, lipid droplet, and mitophagy gene expression of Wistar rat’s liver after injection of streptozotocin (STZ).Materials and Methods: Twenty male Wistar rats were divided into 4 groups: Control (n=5); 100 mg/kg BW/day mitoTEMPOL orally (n=5); 50 mg/kg BW STZ intraperitoneal injection (n=5); and mitoTEMPOL+STZ (n=5). STZ was given a single dose, while mitoTEMPOL was given for 5 weeks after 1 week of STZ injection. Histopathological appearance, lipid droplets, mitophagy, and autophagy gene expression were examined after the mitoTEMPOL treatment. Results: We found metabolic zone shifting that might be correlated with the liver activity of fatty acid oxidation in the STZ group, a decrease of lipid droplets in mitoTEMPOL and mitoTEMPOL + STZ compared with Control and STZ groups were found in this study. We also found significant changes in PINK1, Parkin, BNIP3, Mfn1, and LC3 gene expression, but no difference in Opa1, Fis1, Drp1, and p62 gene expression, suggesting a change of mitochondrial fusion rather than mitochondrial fission correlated with mitophagy.Conclusion: All this concluded that mitoTEMPOL could act as a modulator of mitophagy and metabolic function of the liver, thus amplifying its crucial role in preventing mitochondrial damage in the liver in the early onset of diabetes mellitus. UR - https://ijbms.mums.ac.ir/article_21092.html L1 - https://ijbms.mums.ac.ir/article_21092_451dd7108c835f8b67a09b5a812967ad.pdf ER -