TY - JOUR ID - 21876 TI - LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Deng, Xiaoyu AU - He, Jinke AU - Xu, Jinfeng AU - Wang, Yueli AU - Yi, Jihai AU - Zhang, Huan AU - Wang, Yong AU - Wang, Zhen AU - Chen, Chuangfu AD - School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China AD - Department of Basic Medicine, Xinjiang Second Medical College, Kelamayi, Xinjiang, China AD - Collaborative Innovation Center for Prevention and Control of High Incidence Zoonotic Infectious Diseases in Western China, College of Animal Science and Technology, Shihezi University, Shihezi, China Y1 - 2023 PY - 2023 VL - 26 IS - 5 SP - 564 EP - 571 KW - Adjuvant KW - Brucella KW - Immunogenicity KW - Outer-membrane vesicles Vaccine DO - 10.22038/ijbms.2023.67394.14775 N2 - Objective(s): Existing Brucella vaccines are attenuated and can cause vaccine-associated brucellosis; and these safety concerns have affected their application. Although subunit vaccines have the advantages of safety, efficacy, low cost, and rapid production, they are usually poorly immunogenic and insufficient to trigger persistent immunity. Therefore, we added layered double hydroxide (LDH) as an adjuvant to Brucella subunit vaccine formulations to enhance the immune response to the antigen.Materials and Methods: LDH and Freund’s adjuvant were combined with Brucella outer-membrane vesicles (OMVs) and OMV-associated proteins to form a subunit vaccine, respectively. The immunogenicity of LDH as an adjuvant was assessed in BALB/c mice. We examined levels of immunoglobulin G, G1, and G2a (IgG, IgG1, and IgG2a) antibodies (aBs); percentages of Cluster of Differentiation 4-positive (CD4+) and CD8+ T cells in peripheral-blood lymphocytes; and secretion of cytokines in mouse spleen lymphocytes. Finally, splenic index and splenic bacterial load were assessed via Brucella challenge experiments on mice.Results: The LDH subunit vaccine also produced high levels of specific aBs in mice compared with Freund’s adjuvant subunit vaccine and induced mainly T-helper 1 cell (Th1)-type immune responses. In addition, mice in the LDH subunit vaccine group had significantly lower bacterial loads in their spleens than those in the Freund’s adjuvant subunit vaccine group, and the LDH-OMV vaccine offered a higher level of protection against Brucella attack.Conclusion: LDH as an adjuvant-paired vaccine provided a high level of protection against Brucella infection. UR - https://ijbms.mums.ac.ir/article_21876.html L1 - https://ijbms.mums.ac.ir/article_21876_e398bdff729076b26ca9c73e82b3c0e2.pdf ER -