TY - JOUR ID - 2931 TI - Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Zhao, Jingkun AU - Duan, Shurong AU - Zhou, Jinxia AU - Sun, Ruihong AU - Zhang, Liming AU - Wang, Desheng AD - Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China AD - PuRen Hospital of WuHan, Wu han, China Y1 - 2014 PY - 2014 VL - 17 IS - 6 SP - 454 EP - 459 KW - Cerebral ischemia/reperfusion KW - Fas KW - FasL KW - Hypothermia KW - MACO rat KW - MMP-3 DO - 10.22038/ijbms.2014.2931 N2 - Objective(s):To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods:Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. Immunohistochemistry was performed to determine expression Fas, FasL, and MMP-3. Results: Infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (P<0.05). In mild hypothermia group, numbers of Fas-positive and MMP-3 positive cells were significantly less than those of normothermia group (P<0.05). Neurological functional scores of mild hypothermia were significantly improved (P<0.05). Conclusion: Mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces Fas and MMP-3 expression, but increases FasL in cerebral ischemia-reperfusion rats. UR - https://ijbms.mums.ac.ir/article_2931.html L1 - https://ijbms.mums.ac.ir/article_2931_b923cc1f5f757de29207887f9de1a18c.pdf ER -