TY - JOUR ID - 3709 TI - Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ashrafi Tamai, Iradj AU - Zahraei Salehi, Taghi AU - Sharifzadeh, Aghil AU - Shokri, Hojjatollah AU - Khosravi, Ali Reza AD - Bu Ali Sina University, Hamedan, Iran Research and Training Center of Imam Khomeini Hospital, Tehran, Iran AD - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran Y1 - 2014 PY - 2014 VL - 17 IS - 11 SP - 831 EP - 835 KW - Candida albicans KW - candidiasis KW - Genotyping KW - HIV+ KW - RPS sequence DO - 10.22038/ijbms.2014.3709 N2 - Objective(s): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV+) with or/and without oropharyngeal candidiasis (OPC). Materials and Methods:A total of 100 C. albicans isolates from Iranian HIV+patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. Results: The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. Conclusion:In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV+ patients. UR - https://ijbms.mums.ac.ir/article_3709.html L1 - https://ijbms.mums.ac.ir/article_3709_1d53de43b0eae04be19278bfe8915f4f.pdf ER -