TY - JOUR ID - 3747 TI - Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Sadeghi Fazel, Fariba AU - Haddadi, Mahnaz AU - Khoshnevisan, Alireza AU - Muhammadnejad, Samad AU - Muhammadnejad, Ahad AU - Mazaheri, Zohreh AU - Arjomandnejad, Motahareh AU - Shirkoohi, Reza AU - Oghabian, Mohammad-Ali AU - Sherkat-Khameneh, Narjes AU - Amanpour, Saeid AU - Kazemimanesh, Monireh AD - Health Management Department, Quality Assurance Deputy, Razi Vaccine & Serum Research Institute, Karaj, Iran AD - Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran AD - Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran AD - Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran AD - Anatomy Department, School of Medicine, Tarbiat Modares University, Tehran, Iran AD - Omid Institute for Advance Biomodels, Incubation Center for Medical Devices, Tehran University of Medical Sciences, Tehran, Iran AD - Virology Department, Pasteur Institute of Iran, Tehran, Iran Y1 - 2014 PY - 2014 VL - 17 IS - 11 SP - 922 EP - 925 KW - Glioblastomamultiform KW - In vitro chemosensitivity and resistance assay KW - Personalized Medicine KW - Primary KW - explant technique KW - Stereotactic biopsy DO - 10.22038/ijbms.2014.3747 N2 - Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] . Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods. Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs. UR - https://ijbms.mums.ac.ir/article_3747.html L1 - https://ijbms.mums.ac.ir/article_3747_18889a2b2c356f4ab92fad027b03e2be.pdf ER -