TY - JOUR ID - 3888 TI - In silico and in vitro studies of cytotoxic activity of different peptides derived from vesicular stomatitis virus G protein JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ghandehari, Fereshte AU - Behbahani, Mandana AU - Pourazar, Abbasali AU - Noormohammadi, Zahra AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran AD - Department of Immunology, Isfahan University of Medical Science, Isfahan, Iran Y1 - 2015 PY - 2015 VL - 18 IS - 1 SP - 47 EP - 52 KW - ANTICP KW - Apoptosis KW - Cytotoxic KW - Pseudo typing KW - VSVG protein DO - 10.22038/ijbms.2015.3888 N2 - Objective(s):This study aims at exploring cytotoxic activity of different peptides derived from VSVG protein against MCF-7 and MDA-MB-231 breast cancer cell lines and human embryonic kidney normal cell (HEK 293).    Materials and Methods: The ANTICP web server was used to predict anticancer peptides. The cytotoxic activity of peptides with high score (P26, P7) and low score (P19) was examined by MTT and DNA fragmentation assays. Results: The results obtained from ANTICP web serverdemonstrated that 4 out of 48 peptides (P26, P7, P10, and P16) had anticancer activity. P26 and P7 peptides of these 4 peptides were detected to have high cytotoxic activity against MCF-7 cells with CC50 values of 98,280 µg/ml and MDA-MB231 cells with CC50 100,550 µg/ml, respectively. In addition, the results showedthat amino acid residues of these 4 peptides were located near fusion domain. Conclusion: The results confirmed that P26 and P7 peptides might induce membrane damage and initiate apoptosis. The present study suggested that P26 and P7 peptides could be appropriate candidates for further studies as cytotoxic agents and modifications in the residue at positions 70-280 might potentially produce a more efficient VSVG protein in gene therapy. UR - https://ijbms.mums.ac.ir/article_3888.html L1 - https://ijbms.mums.ac.ir/article_3888_92f764299dfe5ec21c4588d8c48ae616.pdf ER -