TY - JOUR ID - 4026 TI - An in vivo and in vitro investigation on hepatoprotective effects of Pimpinella anisum seed essential oil and extracts against carbon tetrachloride-induced toxicity JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Jamshidzadeh, Akram AU - Heidari, Reza AU - Razmjou, Mojtaba AU - Karimi, Forouzan AU - Moein, Mahmood Reza AU - Farshad, Omid AU - Akbarizadeh, Amin Reza AU - Houshangi Shayesteh, Mohammad Reza AD - Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran AD - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Gerash Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Gerash, Iran AD - Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran AD - Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - Shiraz University of Medical Sciences, International Branch, Shiraz, Iran AD - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2015 PY - 2015 VL - 18 IS - 2 SP - 205 EP - 211 KW - Anise seed KW - CCl4 KW - Hepatotoxicity KW - HepG2 cells KW - Rat KW - Oxidative stress DO - 10.22038/ijbms.2015.4026 N2 - Objective(s):  Protective effects of different extracts and essential oil from Pimpinella anisum           L. seeds were examined against carbon tetrachloride (CCl4)-induced toxicity. The parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. In the in vitro model of this study, markers such as cell viability, cellular reduced and oxidized glutathione and lipid peroxidation in HepG2 cells were evaluated.  Materials and Methods:Human liver cancer cell line HepG2 and male Sprague-Dawley rats were treated with extracts and essential oil, and markers of hepatotoxicity were investigated. Results: The data revealed that the n-hexane extract, effectively attenuated CCl4‑induced toxicity in both in vitro and in vivo models in current investigation. Conclusion: As the oxidative stress markers were ameliorated, it might be concluded that anise seed possesses protective effects probably due to its antioxidant constituents. UR - https://ijbms.mums.ac.ir/article_4026.html L1 - https://ijbms.mums.ac.ir/article_4026_1246bb0b94bb3b9e1aedb76989e73f32.pdf ER -