TY - JOUR ID - 4135 TI - Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Kaeidi, Ayat AU - Taati, Majid AU - Hajializadeh, Zahra AU - Jahandari, Faranak AU - Rashidipour, Marzieh AD - Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran AD - Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran AD - Young Researchers Club, Khoramabad Brach, Islamic Azad University, Khoramabad, Iran Y1 - 2015 PY - 2015 VL - 18 IS - 3 SP - 301 EP - 306 KW - Apoptosis KW - Diabetes KW - Neuropathy KW - Ziziphus Jujube DO - 10.22038/ijbms.2015.4135 N2 - Objective(s):The neuroprotective effect offruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC12 cells as an appropriate in vitro model of diabetic neuropathy was investigated. Materials and Methods: Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-3, a biochemical parameter of cellular apoptosis, was measured by western blot analysis. Results: Our data showed that a 4-fold elevation in glucose levels within the medium significantly reduced cell viability, increased intracellular ROS and caspase-3 activation in PC12 cells after 24 hr. Incubation of the high glucose medium cells with 300-μg/ml Z. jujuba fruit (ZJF) extract decreased the high glucose-induced cell toxicity and prevented caspase-3 activation and excited ROS generation. Conclusion: Thus, we concluded that the aqueous extract of Z. jujuba protects against hyperglycaemia-induced cellular toxicity. This could be associated with the prevention of ROS generation and neural apoptosis. Moreover, the results suggest that the ZJF has a therapeutic potential to attenuate diabetes complications such as neuropathy. UR - https://ijbms.mums.ac.ir/article_4135.html L1 - https://ijbms.mums.ac.ir/article_4135_af86ee14a70c5e4f678d57b22aa6ca70.pdf ER -