TY - JOUR ID - 4649 TI - Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Mansouri Motlagh, Bahman AU - Afzale Ahangaran, Nahideh AU - Abtahi Froushani, Seyyed Meysam AD - Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran Y1 - 2015 PY - 2015 VL - 18 IS - 7 SP - 672 EP - 676 KW - Calcitriol KW - Macrophage KW - Mesenchymal stem cell DO - 10.22038/ijbms.2015.4649 N2 - Objective(s):Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet.  The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages. Materials and Methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.  Results: Our data showed that bonemarrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group. Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs UR - https://ijbms.mums.ac.ir/article_4649.html L1 - https://ijbms.mums.ac.ir/article_4649_844bce7559fd2925f85695f8204a50c3.pdf ER -