TY - JOUR ID - 4978 TI - Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Eftekhar, Fereshteh AU - Dadaei, Taraneh AD - Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Evin, Tehran, Iran Y1 - 2011 PY - 2011 VL - 14 IS - 2 SP - 132 EP - 136 KW - biofilm formation KW - IcaAB KW - MRSA KW - Staphylococcus aureus DO - 10.22038/ijbms.2011.4978 N2 - Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less well described in S. aureus. We studied biofilm formation in clinical isolates of MRSA in relation to the presence of the ica operon. Materials and Methods Forty five MRSA were studied for biofilm formation by colony morphology on Congo red agar (CRA) and the microtitre plate assay (MtP). Presence of the ica genes was detected by PCR and specific primers. Results The results showed that 53.3% of the isolates had the potential to form biofilm by colony morphology of which, 75% carried the ica operon. Weak biofilm production was observed in the MtP assay by 57.8%, of which 53.8% harbored the ica operon. However, about 70% of biofilm non-producers also carried the ica operon. Conclusion Overall, there was no agreement between the icaAB gene carriage and biofilm phenotype by either of the two phenotypic methods. However, 91% of biofilm formers on CRA also produced biofilm in the MtP assay. UR - https://ijbms.mums.ac.ir/article_4978.html L1 - https://ijbms.mums.ac.ir/article_4978_fe9ec81b22536a4cd4c65323c450324e.pdf ER -