TY - JOUR ID - 6409 TI - Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Bidkhori, Hamid Reza AU - Ahmadiankia, Naghmeh AU - Moghaddam Matin, Maryam AU - Heirani tabasi, Asieh AU - Farshchian, Moein AU - Naderi-meshkin, Hojjat AU - Shahriyari, Mina AU - Dastpak, Mahtab AU - Bahrami, Ahmad Reza AD - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran AD - Shahroud University of Medical Sciences, Shahroud, Iran AD - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran AD - Stem Cells and Regenerative Medicine Research Department, ACECR-Khorasan Razavi Branch, Mashhad, Iran Y1 - 2016 PY - 2016 VL - 19 IS - 1 SP - 14 EP - 19 KW - CXCR4 KW - CXCR7 KW - CoCl2 KW - Desferrioxamine KW - MSC KW - Chemical treatment KW - Homing KW - Valproic acid DO - 10.22038/ijbms.2016.6409 N2 - Objective(s):The limited homing potential of bone-marrow-derived mesenchymal stem cells (BM-MSC) is the key obstacle in MSC-based therapy. It is believed that chemokines and chemokine receptor interactions play key roles in cellular processes associated with migration. Meanwhile, MSCs express a low level of distinct chemokine receptors and they even lose these receptors on their surface after a few passages which influence their therapeutic applications negatively. This study investigated whether treatment of BM-MSCs with hypoxia-mimicking agents would increase expression of some chemokine receptors and cell migration. Materials and Methods: BM-MSCs were treated at passage 2 for our gene expression profiling. All qPCR experiments were performed by SYBR Green method in CFX-96 Bio-Rad Real-Time PCR. The Boyden chamber assay was utilized to investigate BM-MSC homing. Results:Possible approaches to increasing the expression level of chemokine receptors by different hypoxia-mimicking agents such as valproic acid (VPA), CoCl2, and desferrioxamine (DFX) are described. Results show DFX efficiently up-regulate the CXCR7 and CXCR4 gene expression while VPA increase only the CXCR7 gene expression and no significant change in expression level of CXCR4 and the CXCR7 gene was detectable by CoCl2 treatment. Chemotaxis assay results show that pre-treatment with DFX, VPA, and Cocl2 enhances significantly the migration ability of BM-MSCs compared with the untreated control group and DFX treatment accelerates MSCs homing significantly with a higher rate than VPA and Cocl2 treatments. Conclusion: Our data supports the notion that pretreatment of MSC with VPA and DFX improves the efficiency of MSC therapy by triggering homing regulatory signaling pathways. UR - https://ijbms.mums.ac.ir/article_6409.html L1 - https://ijbms.mums.ac.ir/article_6409_eace9d6f07c7fd009845ce6e5b4fe7ab.pdf ER -