TY - JOUR ID - 6412 TI - Laminin matrix promotes hepatogenic terminal differentiation of human bone marrow mesenchymal stem cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Khalaj, Zahra AU - Sahebghadam Lotfi, Abbas AU - Kabir-Salmani, Maryam AD - Animal and Marine Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AD - Biomaterials and Tissue Engineering Department, Stem Cell Division, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Y1 - 2016 PY - 2016 VL - 19 IS - 1 SP - 34 EP - 42 KW - bone marrow KW - Differentiation KW - Hepatocyte KW - Laminin KW - Mesenchymal stem cell DO - 10.22038/ijbms.2016.6412 N2 - Objective(s):The application of stem cells holds great promises in cell transplants. Considering the lack of optimal in vitro model for hepatogenic differentiation, this study was designed to examine the effects of laminin matrix on the improvement of in vitro differentiation of human bone marrow mesenchymal stem cells (hBM-MSC) into the more functional hepatocyte-like cells. Materials and Methods:Characterization of the hBM-MSCs was performed by immunophenotyping and their differentiation into the mesenchymal-derived lineage. Then, cells were seeded on the laminin-coated or tissue culture polystyrene (TCPS). The differentiation was carried out during two steps. Afterward, the expression of hepatocyte markers such as AFP, ALB, CK-18, and CK-19 as well as the expression of C-MET, the secretion of urea, and the activity of CYP3A4 enzyme were determined. Moreover, the cytoplasmic glycogen storage was examined by periodic acid–Schiff (PAS) staining. Results:The results demonstrated that the culture of hBM-MSC on laminin considerably improved hepatogenic differentiation compared to TCP group. A significant elevated level of urea biosynthesis and CYP3A4 enzyme activity was observed in the media of the laminin-coated differentiated cells (P<0.05). Furthermore higher expressions of both AFP and ALB were determined in cells differentiated on laminin matrix. Glycogen accumulation was not detected in the undifferentiated hBM-MSCs, however, both differentiated cells in laminin and TCPS groups demonstrated the intracellular glycogen accumulation on day 21 of hepatogenic differentiation. Conclusion:Taken together, these findings may indicate that laminin matrix can improve terminal differentiation of hepatocyte-like cells from hBM-MSCs. Thus, laminin might be considered as a suitable coating in hepatic tissue engineering designs. UR - https://ijbms.mums.ac.ir/article_6412.html L1 - https://ijbms.mums.ac.ir/article_6412_1a1645fd8d0d8126bd09b31990d9e71e.pdf ER -