TY - JOUR ID - 6648 TI - The effect of omega- 3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Krizak, Jakub AU - Frimmel, Karel AU - Bernatova, Iveta AU - Navarova, Jana AU - Sotnikova, Ruzena AU - Okruhlicova, Ludmila AD - Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia AD - Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovakia AD - Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia Y1 - 2016 PY - 2016 VL - 19 IS - 3 SP - 290 EP - 299 KW - aorta KW - Endothelium KW - Lipopolysaccharide KW - Occludin KW - Omega-3 polyunsaturated fatty acids DO - 10.22038/ijbms.2016.6648 N2 - Objective(s): Occludin is essential for proper assembly of tight junctions (TJs) which regulate paracellular endothelial permeability. Omega-3 polyunsaturated fatty acids (Ω-3 PUFA) protect endothelial barrier function against injury. Materials and Methods: We examined anti-inflammatory effect of Ω-3 PUFA intake (30 mg/kg/day for 10 days) on expression and location of occludin in the aorta of adult Wistar rats after a single dose of bacterial lipopolysaccharide (LPS, Escherichia coli, 1 mg/kg). The ultrastructure of TJs after LPS administration was also investigated. We measured plasma levels of C-reactive protein (CRP), Malondialdehyde (MDA) and CD68 expression and determined the total activity of NO synthase (NOS) in the aortic tissue. Results:LPS induced a significant decrease of occludin expression accompanied by structural alterations of TJs. Levels of CRP, MDA, CD68 and NOS activity were elevated after LPS injection compared to controls indicating presence of moderate inflammation. Ω-3 PUFA supplementation did not affect occludin expression in treated inflammatory group. However they reduced CRP and MDA concentration and CD68 expression, but conversely, they increased NOS activity compared to inflammatory group. Conclusion:Our results indicate that a single dose of LPS could have a long-term impact on occludin expression and thus contribute to endothelial barrier dysfunction. 10-day administration of Ω-3 PUFA had partial anti-inflammatory effects on health of rats without any effect on occludin expression. UR - https://ijbms.mums.ac.ir/article_6648.html L1 - https://ijbms.mums.ac.ir/article_6648_d3faf8bcd9cdda421d304d5d6b9a4604.pdf ER -