TY - JOUR ID - 6943 TI - Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Mei, Yang AU - Yangyang, Zhuang AU - Shuai, Luo AU - Hao, Jin AU - Yirong, Yang AU - Yong, Cai AU - Peng, Xia AU - Bicheng, Chen AU - Yan, Zhang AD - Department of Intensive Care Unit, The First Affiliate Hospital of Wenzhou Medicine University, China AD - Department of Transplantation Center, the First Affiliate Hospital of Wenzhou Medicine University, China AD - Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital of Wenzhou Medicine University, China Y1 - 2016 PY - 2016 VL - 19 IS - 5 SP - 573 EP - 578 KW - Antioxidant KW - Biological properties KW - HPLC KW - RSM KW - Stachys parviflora L KW - UAE DO - 10.22038/ijbms.2016.6943 N2 - Objective(s):Our recent report indicates that breviscapine play a protective role of the kidney by down-regulating transforming growth factor-β1(TGF-β1), α-smooth muscle actin (α-SMA) and alleviating interstitial fibrosis following unilateral ureteral obstruction (UUO). In this study, we investigate the effect of breviscapine on changes of renal water and sodium transport proteins in response to UUO. Materials and Methods: Male Sprague-Dawley rats were divided into 3 groups, sham group, UUO group and UUO treat with breviscapine. After 4, 7 and 14 days, histologic changes and interstitial collagen were determined microscopically following hematoxylin and eosin (H&E) and Masson's trichrome staining. The expression of Aquaporins (AQP-2) and γ-epithelial sodium channel (γ-ENaC) were investigated using immunohistochemistry and Western blot in each group. Results:Breviscapine treatment decrease the tubular injury index and the degree of interstitial collagen deposition significantly compared with the UUO group (P<0.05). Breviscapine treatment also significantly reduced downregulation of AQP2 and γ-ENaC compared to those subjected to the same time course of obstruction in UUO group (P<0.05).  Conclusion:These results demonstrate that breviscapine could prevent downregulation of renal water and sodium transport proteins in response to UUO so as to protect obstructed kidney. UR - https://ijbms.mums.ac.ir/article_6943.html L1 - https://ijbms.mums.ac.ir/article_6943_2f21c7cc6919793983d8d7190c6cdb99.pdf ER -