TY - JOUR ID - 7351 TI - Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Bi, Miao-Miao AU - Hong, Sen AU - Ma, Ling-Jun AU - Zhou, Hong-Yan AU - Lu, Jia AU - Zhao, Jing AU - Zheng, Ya-Juan AD - Department of Ophthalmology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin, 130041, P.R. China AD - Department of Colon and Anal Surgery, the First hospital of Jilin University, Jilin University, Changchun, Jilin, 130021, P.R. China AD - Department of Ophthalmology, The China-Japan Union Hospitial of Jilin University, Jilin University, Changchun, Jilin, 130033, Xiantai Street No. 126, Jilin Province, China Y1 - 2016 PY - 2016 VL - 19 IS - 7 SP - 705 EP - 711 KW - Apoptosis KW - Chloride channels KW - Chloride channel protein 2 Neuroprotection KW - Retinal ganglion cells DO - 10.22038/ijbms.2016.7351 N2 - Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Caspase-3 and -9 activities were determined by a colorimetric assay. The roles of ClC-2 in glutamate-induced apoptosis were examined by using ClC-2 complementary deoxyribonucleic acid (cDNA) and small inference ribonucleic acid (RNA) transfection technology. Results: Overexpression of ClC-2 in RGC-5 cells significantly decreased glutamate-induced apoptosis and increased cell viability, whereas silencing of ClC-2 with short hairpin (sh) RNA produced opposite effects. ClC-2 overexpression increased the expression of Bcl-2, decreased the expression of Bax, and decreased caspase-3 and -9 activation in RGC-5 cells treated with glutamate, but silencing of ClC-2 produced opposite effects. Conclusion: Our data suggest that ClC-2 chloride channels might play a protective role in glutamate-induced apoptosis in retinal ganglion cells via the mitochondria-dependent apoptosis pathway. UR - https://ijbms.mums.ac.ir/article_7351.html L1 - https://ijbms.mums.ac.ir/article_7351_dcb4fcc962af55efa7e1ce2bdce6db24.pdf ER -