TY - JOUR ID - 7356 TI - Loss of heterozygosity and microsatellite instability as predictive markers among Iranian esophageal cancer patients JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Forghanifard, Mohammad Mahdi AU - Emami Vahid, Elham AU - Dadkhah, Ezzat AU - Gholamin, Mehran AU - Broumand Noghabi, Samaneh AU - Ghahraman, Martha AU - Farzadnia, Mehdi AU - Abbaszadegan, Mohammad Reza AD - Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran AD - Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran AD - School of Systems Biology, George Mason University, Manassas, Virginia, U.S.A AD - Department of hematopathology, Central Laboratory, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Cancer Molecular Pathology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2016 PY - 2016 VL - 19 IS - 7 SP - 726 EP - 733 KW - Esophageal squamous cell -carcinoma KW - Loss of heterozygosity KW - Microsatellite instability DO - 10.22038/ijbms.2016.7356 N2 - Objective(s): Variation in microsatellite sequences that are dispersed in the genome has been linked to a deficiency in cellular mismatch repair system and defects in several genes of this system are involved in carcinogenesis. Our aim in this study was to illustrate microsatellite DNA alteration in esophageal cancer. Materials and Methods: DNA was extracted from formalin fixed paraffin embedded (FFPE) tissues from surgical and matched margin-normal samples. Microsatellite instability (MSI) and loss of heterozygosity (LOH) were studied in 50 cases of esophageal squamous cell carcinoma (ESCC) by amplifying six microsatellite markers: D13S260 (13q12.3), D13S267 (13q12.3), D9S171 (9p21), D2S123 (2p), D5S2501 (5q21) and TP53 (17p13.1) analyzed on 6% denaturing polyacrylamide gel electrophoresis. Results: Statistical analysis indicated a near significant reverse correlation between grade and LOH (P= 0.068, correlation coefficient= -0.272). Specifically, increased LOH in tumor DNA has a significant correlation with increased differentiation from poorly differentiated to well differentiated tumors (P= 0.002 and P= 0.016 respectively). In addition, higher number of chromosomal loci with LOH showed a reverse correlation with lymph node metastasis (P= 0.026, correlation coefficient= -0.485). Furthermore, there was a positive correlation between addiction and MSI (P= 0.026, correlation coefficient= 0.465). Conclusion: Microsatellite DNA alterations may be a prognostic tool for detection and the evolution of prognosis in patients with SCC of esophagus. It can be concluded that regional lymph node metastasis would be less likely with increased heterozygote loci and addiction with any of opium, cigarette, water pipe or alcohol can be a susceptibility factor(s) for MSI. UR - https://ijbms.mums.ac.ir/article_7356.html L1 - https://ijbms.mums.ac.ir/article_7356_09e6f16f2c5afde2adc9d97dfc5c4f02.pdf ER -