TY - JOUR ID - 8589 TI - Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenic Escherichia coli JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Shakerimoghaddam, Ali AU - Ghaemi, Ezzat A AU - Jamalli, Ailar AD - Department of Microbiology, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran AD - Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 4 SP - 451 EP - 456 KW - Biofilm KW - Urinary tract infection KW - Uropathogenic Escherichia coli KW - Zinc oxide nanoparticle DO - 10.22038/ijbms.2017.8589 N2 - Objective(s): This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-np) on biofilm formation and expression of the flu gene in uropathogenic Escherichia coli (UPEC) strains. Materials and Methods: Minimum inhibitory concentration (MIC) of ZnO-np was determined by agar dilution method. The effect of MIC and sub-MIC concentrations of ZnO-np on biofilm formation were determined by microtiter plate assay. The expression level of the flu gene was assessed by Real-Time PCR assay. Results: MIC and sub-MIC ZnO-np concentrations reduced biofilm formation by 50% and 33.4%, respectively. Sub-MIC ZnO-np concentration significantly reduced the flu gene expression in the UPEC isolates (P<0.0001). Conclusion: The sub-MIC concentration of ZnO-np reduces biofilm formation and flu gene expression in UPEC isolates. It is suggested to use nanoparticles for coating medical devices to prevent bacterial colonization. UR - https://ijbms.mums.ac.ir/article_8589.html L1 - https://ijbms.mums.ac.ir/article_8589_156514a99b5b33e11cde95765dcec9f2.pdf ER -