TY - JOUR ID - 990 TI - Prevalence of PER and VEB Type Extended Spectrum Betalactamases among Multidrug Resistant Acinetobacter baumannii Isolates in North-West of Iran JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Farajnia, Safar AU - Azhari, Fatemeh AU - Alikhani, Mohammad Yousef AU - Hosseini, Mohammad Kazem AU - Peymani, Amir AU - Sohrabi, Nasrolah AD - Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran AD - Biotechnology Research Center, Tabriz University of Medical Science, Tabriz, Iran AD - Microbiology Department, Hamadan University of Medical Science, Hamadan, Iran AD - Istanbul University, Faculty of Sciences, Molecular Biology and Genetic, Istanbul, Turkey AD - Microbiology Department, Qazvin University of Medical Science, Qazvin, Iran AD - Paramedical Faculty, Kermanshah University of Medical Sciences, Kermanshah, Iran Y1 - 2013 PY - 2013 VL - 16 IS - 6 SP - 751 EP - 755 KW - Acinetobacter baumannii Extended spectrum betalactamases Integron class I DO - 10.22038/ijbms.2013.990 N2 -   Objective(s): Drug resistant Acinetobacter baumannii have emerged as a major problem in many hospitals and intensive care units. Various types of extended spectrum beta-lactamases (ESBLs) are responsible for resistance to beta- lactam antibiotics in different parts of the world. The objective of this study was to determine the prevalence of integron class1 (INT 1) and ESBL types PER-1, PER-2 and VEB-1 among A. baumannii strains isolated from Tabriz, North-West of Iran.   Material and Methods: A total of 100 A. baumannii isolates collected from different clinical samples were included in the study. Antimicrobial susceptibility profiles were determined using the Kirby Bauer disk diffusion method. Production of ESBL was investigated by testing resistance against ceftazidime, cefotaxime, ceftriaxone and verified by Double Disk Synergy Test. DNA was extracted from the isolates and the frequency of INT 1 and ESBL types PER-1, PER-2 and VEB-1 were determined by PCR using specific primers. Results: Among 100 A. baumannii isolates screened, 80 isolates were multidrug-resistant and 70 isolates were positive for ESBL production. PCR screening revealed that 74 % of the isolates contained class 1 integron, 51% were positive for PER-1 gene, 10% positive for VEB1 whereas none of the isolates were positive for PER2 type gene. Conclusion: This is the first report of ESBL types VEB and PER in A. baumannii from North West of Iran. The results of this study demonstrated high prevalence of PER-1 and VEB-1 type ESBLs among A. baumannii isolates in the study region and reminded the necessity of appropriate infection control strategy to prevent further spread of infection by these organisms. UR - https://ijbms.mums.ac.ir/article_990.html L1 - https://ijbms.mums.ac.ir/article_990_62b06cee9baf051e8cc84e1594eec470.pdf ER -