<?xml version="1.0" encoding="utf-8"?>
			<journal>
			<title>Iranian Journal of Basic Medical Sciences</title>
			<title_fa></title_fa>
			<short_title></short_title>
			<subject>Medical Sciences</subject>
			<web_url>https://ijbms.mums.ac.ir/</web_url>
			<journal_hbi_system_id>0</journal_hbi_system_id>
			<journal_hbi_system_user></journal_hbi_system_user>
			<journal_id_issn>2008-3866</journal_id_issn>
			<journal_id_issn_online>2008-3874</journal_id_issn_online>
			<journal_id_pii></journal_id_pii>
			<journal_id_doi></journal_id_doi>
			<journal_id_iranmedex></journal_id_iranmedex>
			<journal_id_magiran></journal_id_magiran>
			<journal_id_sid></journal_id_sid>
			<journal_id_nlai></journal_id_nlai>
			<journal_id_science></journal_id_science>
			<language>en</language>
			<pubdate>
				<type>jalali</type>
				<year>0</year>
				<month>0</month>
				<day>1</day>
			</pubdate>
			<pubdate>
				<type>gregorian</type>
				<year>2019</year>
				<month>1</month>
				<day>1</day>
			</pubdate>
			<volume>22</volume>
			<number>1</number>
			<publish_type>online</publish_type>
			<publish_edition>1</publish_edition>
			<article_type>fulltext</article_type>
			<articleset><article>
				<language>en</language>
				<article_id_issn></article_id_issn>
				<article_id_issn_online></article_id_issn_online>
				<article_id_pubmed></article_id_pubmed>
				<article_id_pii></article_id_pii>
				<article_id_doi></article_id_doi>
				<article_id_iranmedex></article_id_iranmedex>
				<article_id_magiran></article_id_magiran>
				<article_id_sid></article_id_sid>
				<title_fa></title_fa>
				<title>Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China</title>
				<subject_fa></subject_fa>
				<subject></subject>
				<content_type_fa></content_type_fa>
				<content_type>Short Communication</content_type>
				<abstract_fa><![CDATA[]]></abstract_fa>
				<abstract><![CDATA[Objective(s): Acinetobacter pittii has become an emerging opportunistic noscomial pathogen worldwide with multi-drug resistance. In the present study, an A. pittii strain was isolated from bronchoalveolar lavage fluid sample harboring both OXA-58 and NDM-1carbapenemase producing genes. The mechanisms of carbapenem resistance of the A. pittii strain was investigated.Materials and Methods: Carbapenemase producing genes were examined by PCR and DNA sequencing. S1-PFGE was used to localize carbapenemase encoding genes. Filter mating and electrotransformation were used to investigate the transferability of such carbapenemase encoding genes between different strains. Genetic surroundings of blaOXA-58 and blaNDM-1 genes were detected as well.Results: The A. pittii strain, carrying both OXA-58 and NDM-1 carbapenemase encoding genes, was resistant to all β-lactam antibiotics, while suscepitible to ciprofloxacin, levofloxacin, tobramycin, cotrimoxazole and tigecycline. Southern blot hybridization for the blaOXA-58 and blaNDM-1 gene indicated that the two genes locate in the same plasmid with molecular weight of 310.1-336.5kb. BlaOXA-58 was located in an ISAba3-blaOXA-58-ISAba3-like structure, and the blaNDM-1 gene cluster was embedded in an ISAba125-aphA6- blaNDM-1-bleMBL-ΔtrpF-dsbC-cutA structure sequentially.Conclusion: In the present study, it is first reported an A. pittii clincal strain in China, co-harboring OXA-58 and NDM-1 carbapenemase producing genes residing on a same plasmid. In hospital and community settings, it is of great significance and urgence to increase the surveillance of these kinds of organisms.]]></abstract>
				<keyword_fa></keyword_fa>
				<keyword>Acinetobacter pittii, Carbapenemase, Co-harboring, NDM-1, OXA-58</keyword>
				<start_page>106</start_page>
				<end_page>111</end_page>
				<web_url>https://ijbms.mums.ac.ir/article_11601.html</web_url>
			<author_list><author>
				<first_name>Yili</first_name>
				<middle_name></middle_name>
				<last_name>Chen</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>13570260173@163.com</email>
				<code>48872</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Laboratory Medicine, The First Afﬁliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Penghao</first_name>
				<middle_name></middle_name>
				<last_name>Guo</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>acasia@163.com</email>
				<code>48873</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Laboratory Medicine, The First Afﬁliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Han</first_name>
				<middle_name></middle_name>
				<last_name>Huang</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>huanghan007@163.com</email>
				<code>48874</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Laboratory Medicine, The First Afﬁliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Yongxin</first_name>
				<middle_name></middle_name>
				<last_name>Huang</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>huangyongxin@163.com</email>
				<code>48875</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Laboratory Medicine, Zhongshan Medical School of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Zhongwen</first_name>
				<middle_name></middle_name>
				<last_name>Wu</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>wuzhongwen@163.com</email>
				<code>48876</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Laboratory Medicine, The First Afﬁliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Kang</first_name>
				<middle_name></middle_name>
				<last_name>Liao</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>nancy20150919@163.com</email>
				<code>48877</code>
				<coreauthor>Yes</coreauthor>
				<affiliation>Department of Laboratory Medicine, The First Afﬁliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author></author_list>
				</article>
			</articleset>
			</journal>