<?xml version="1.0" encoding="utf-8"?>
			<journal>
			<title>Iranian Journal of Basic Medical Sciences</title>
			<title_fa></title_fa>
			<short_title></short_title>
			<subject>Medical Sciences</subject>
			<web_url>https://ijbms.mums.ac.ir/</web_url>
			<journal_hbi_system_id>0</journal_hbi_system_id>
			<journal_hbi_system_user></journal_hbi_system_user>
			<journal_id_issn>2008-3866</journal_id_issn>
			<journal_id_issn_online>2008-3874</journal_id_issn_online>
			<journal_id_pii></journal_id_pii>
			<journal_id_doi></journal_id_doi>
			<journal_id_iranmedex></journal_id_iranmedex>
			<journal_id_magiran></journal_id_magiran>
			<journal_id_sid></journal_id_sid>
			<journal_id_nlai></journal_id_nlai>
			<journal_id_science></journal_id_science>
			<language>en</language>
			<pubdate>
				<type>jalali</type>
				<year>0</year>
				<month>0</month>
				<day>1</day>
			</pubdate>
			<pubdate>
				<type>gregorian</type>
				<year>2017</year>
				<month>4</month>
				<day>1</day>
			</pubdate>
			<volume>20</volume>
			<number>4</number>
			<publish_type>online</publish_type>
			<publish_edition>1</publish_edition>
			<article_type>fulltext</article_type>
			<articleset><article>
				<language>en</language>
				<article_id_issn></article_id_issn>
				<article_id_issn_online></article_id_issn_online>
				<article_id_pubmed></article_id_pubmed>
				<article_id_pii></article_id_pii>
				<article_id_doi></article_id_doi>
				<article_id_iranmedex></article_id_iranmedex>
				<article_id_magiran></article_id_magiran>
				<article_id_sid></article_id_sid>
				<title_fa></title_fa>
				<title>A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies</title>
				<subject_fa></subject_fa>
				<subject></subject>
				<content_type_fa></content_type_fa>
				<content_type>Original Article</content_type>
				<abstract_fa><![CDATA[]]></abstract_fa>
				<abstract><![CDATA[Objective(s): Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in TB diagnosis. Materials and Methods: A fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed. The fusion polyprotein was used as an immunogen to elicit production of multi-target antibodies. Their reactivities were tested. Then, the multi-target antibodies and three corresponding antibodies elicited by each single antigen (mono-target antibodies) were evaluated with sandwich ELISA for detecting M. tuberculosis antigens. Their diagnostic efficacies for TB were also compared. Results: The polyprotein successfully elicited production of multi-target antibodies targeting 38kD, ESAT6, and CFP10 as analyzed by Western blotting. When used as coating antibodies, the multi-target antibodies were more efficient in capturing the three antigens than the corresponding mono-target antibodies. By testing clinical serum, the multi-target antibodies demonstrated significantly higher sensitivity for clinical TB diagnosis than all three mono-target antibodies. Conclusion: The multi-target antibodies allowed detecting multiple antigens simultaneously and significantly enhanced TB detection compared to routine mono-target antibodies. Our study may provide a promising strategy for TB diagnosis.]]></abstract>
				<keyword_fa></keyword_fa>
				<keyword>Antibodies, Antigen, Diagnosis, Tuberculosis, ELISA</keyword>
				<start_page>360</start_page>
				<end_page>367</end_page>
				<web_url>https://ijbms.mums.ac.ir/article_8575.html</web_url>
			<author_list><author>
				<first_name>Zhenhua</first_name>
				<middle_name></middle_name>
				<last_name>Dai</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>44871232@qq.com</email>
				<code>35038</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Zhiqiang</first_name>
				<middle_name></middle_name>
				<last_name>Liu</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>biodiagnosis_liu@163.com</email>
				<code>35039</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Bingshui</first_name>
				<middle_name></middle_name>
				<last_name>Xiu</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>xbssbx@vip.sina.com</email>
				<code>35040</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Xiqin</first_name>
				<middle_name></middle_name>
				<last_name>Yang</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>yangxiqin001@163.com</email>
				<code>35041</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Ping</first_name>
				<middle_name></middle_name>
				<last_name>Zhao</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>zhaopingdiagnosis@163.com</email>
				<code>35042</code>
				<coreauthor>No</coreauthor>
				<affiliation>Chaoyang District Centre for Disease Control and Prevention, 25 Panjiayuan Huaweili, Beijing 100029, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Xuhui</first_name>
				<middle_name></middle_name>
				<last_name>Zhang</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>zhanghhsnow@hotmail.com</email>
				<code>35043</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Cuimi</first_name>
				<middle_name></middle_name>
				<last_name>Duan</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>duancuimi@126.com</email>
				<code>35044</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Haiping</first_name>
				<middle_name></middle_name>
				<last_name>Que</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>qhpp2005@163.com</email>
				<code>35045</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Heqiu</first_name>
				<middle_name></middle_name>
				<last_name>Zhang</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>zhangheqiu2004@126.com</email>
				<code>35046</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Xiaoyan</first_name>
				<middle_name></middle_name>
				<last_name>Feng</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>biodiagnosis2014@163.com</email>
				<code>35047</code>
				<coreauthor>Yes</coreauthor>
				<affiliation>Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author></author_list>
				</article>
			</articleset>
			</journal>