Effect of thymoquinone on vitamin D metabolism in glucocorticoid-induced insulin resistance

Document Type : Original Article

Authors

1 Van Yuzuncu Yil University, Institute of Health Sciences, Van, Turkiye

2 Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Biochemistry Department, Van, Turkiye

10.22038/ijbms.2024.81932.17725

Abstract

Objective(s): The key ingredient in Nigella sativa, thymoquinone (TQ), has several beneficial (antioxidant and anti-inflammatory) properties. This study aimed to investigate the vitamin D metabolism in insulin resistance and the effects of TQ.
Materials and Methods: Male Wistar albino rats were used. TQ was administered as a therapy, and prophylaxis and treatment with metformin were set up for the groups in which insulin resistance had been developed. The gene groups implicated in vitamin D metabolism underwent RT-PCR gene expression analysis and western blot protein analysis.
Results: The analysis shows that the application of TQ reduced HOMA-IR (a sign of insulin resistance). The expression of the VDR gene may be responsible for TQ's effect on treating insulin resistance.
Conclusion: It has been demonstrated that using TQ for therapeutic and preventive reasons is advantageous for improving insulin resistance metrics. Serum vitamin D level was also found to be impacted, which was found to be directly related to the expression of several genes involved in vitamin D metabolism in the liver. However, some of these genes were found to be relatively ineffective in the present study.

Keywords

Main Subjects

References

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Iranian Journal of Basic Medical Sciences
Volume 28, Issue 3
March 2025
Pages 292-298

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