Iranian Journal of Basic Medical Sciences

Iranian Journal of Basic Medical Sciences

The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter 10 coal dust-exposed rats

Authors
Romadhiyana Kisno Saputri Kisno Saputri 1
Bambang Setiawan 2
Dian Nugrahenny 3
Nia Kania 4
Endang Sri Wahyuni 5
M Aris Widodo 3
1 Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
2 Department of Medical Chemistry and Biochemistry, Research Center for Toxicology, Cancer and Degenerative Disease, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
3 Laboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
4 Department of Pathology, Research Center for Toxicology, Cancer and Degenerative Disease, Ulin General Hospital, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
5 Laboratory of Physiology; Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
10.22038/ijbms.2014.3035
Abstract
Objective(s):To investigate the effect of Eucheuma cottonii on alveolar macrophages (AM) and malondialdehyde (MDA) levels in bronchoalveolar lavage fluids (BALF) in particulate matter 10 (PM10) coal dust-exposed rats.
Materials and Methods: Ten groups, including a non exposed group and groups exposed to coal dust at doses of 6.25 (CD6.25), 12.5 (CD12.5), or 25 mg/m3 (CD25) an hour daily for 6 months with or without supplementation of ethanolic extract of E. cottonii at doses of 150 (EC150) or 300 mg/kg BW (EC300). The number of macrophages was determined using a light microscope. MDA levels were measured by TBARS assay.
Results: EC150 insignificantly (P > 0.05) reduces the AM in CD groups compared to non treatment groups. EC150 and EC300 significantly (P < 0.05) decreased MDA levels in CD12.5 and CD25 groups relative to non treatment groups.
Conclusion: E. cottonii attenuated oxidative stress in chronic exposure of PM10 coal dust.
Keywords
Alveolar macrophage
Coal dust
Eucheuma cottonii
Malondialdehyde
  1. Pinho RA, Bonatto F, Andrades M, Frota ML Jr, Ritter C, Klamt F, et al. Lung oxidative response after acute coal dust exposure. Environ Res 2004; 96:290-297.
  2. Forman HJ, Torres M. Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling. Am J Respir Crit Care Med 2002; 166:S4-8.
  3. Lodovici M, Bigagli E. Oxidative stress and air pollution exposure. J Toxicol 2011; 2011. Article ID 487074, 9 pages
  4. Nagarani N, Kamaguru AK. Evaluation of anti-inflammatory, antidiabetic, cytotoxic activity of Kappaphycus alvarezii. Int J Pharm Bio Sci 2013; 4:921-929.
  5. Devi KP, Suganthy N, Kesika P, Pandian SK. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Complement Altern Med 2008; 8:38.
  6. Mohamed S. Healing power of Malaysian seaweeds: cover story. Synth 2011; 32-33:4-5.
  7. Ghasemi Fard S, Tash Shamsabadi F, Emadi M, Meng GY, Muhammad K, Mohamed S. Ethanolic extract of Eucheuma cottonii promotes in vivo hair growth and wound healing. J Anim Vet Adv 2011; 10:601-605.
  8. Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement Altern Med 2008; 8:54.
  9. Kania N, Setiawan B, Widjajanto E, Nurdiana N, Widodo MA, Kusuma HMSC. Peroxidative index as novel marker of hydrogen peroxide involvement in lipid peroxidation from coal dust exposure. Oxid Antioxid Med Sci 2012; 1:209-215.
  10. Curry-McCoy TV, Venado A, Guidot DM, Joshi PC. Alcohol ingestion disrupts alveolar epithelial barrier function by activation of macrophage-derived transforming growth factor beta1. Respir Res 2013; 14:39.
  11. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186:421-431.
  12.  Ghanem MM, Battelli LA, Mercer RR, Scabilloni JF, Kashon ML, Ma JY, et al. Apoptosis and Bax expression are increased by coal dust in the polycyclic aromatic hydrocarbon-exposed lung. Environ Health Perspect 2006; 114:1367-1373.
  13.  Huang YC, Soukup J, Harder S, Becker S. Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage. Am J Physiol Cell Physiol, 2003; 284:24-32.
  14.  Niemiec T, Sawosz E, Grodzik M, Chwalibog A. High L-ascorbic acid content of diet modulates the non-specific immunity in rats. Anim Sci Papers Rep 2009; 27:149-157.
  15. Matanjun P, Mohamed S, Muhammad K, Mustapha NM. Comparison of cardiovascular protective effects of tropical seaweeds, Kappaphycus alvarezii, Caulerpa lentillifera, and Sargassum polycystum, on high-cholesterol/high-fat diet in rats. J Med Food 2010; 13:792-800.