Resveratrol decreases apoptosis and NLRP3 complex expressions in experimental varicocele rat model

Document Type: Original Article

Authors

1 Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran

2 Department of Genetics and Biochemistry, School of Medicine, Arak University of Medical Sciences, Arak, Iran

3 Department of Immunology and Microbiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran

4 Department of Anatomy, School of Medicine, Giulan University of Medical Sciences, Rasht, Iran

5 Institute of Neuroanatomy, Medical Clinic, RWTH Aachen University, 52074 Aachen, Germany

Abstract

Objective(s): Varicocele is an abnormal dilation in the testicular vein, which can cause hypoxia, reactive oxygen species accumulation, elevation in testicular temperature, and promote apoptosis and increase proinflammatory cytokine production. According to the varicocele pathophysiology, it is possible that a group of cytosolic receptors called nucleotide oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasomes also involve in varicocele pathogenesis. Due to the important role of antioxidant in decreasing the testis tissue damage, in this study we investigated the protective effect of resveratrol (RES) on NLRP3 complex and apoptosis in experimental varicocele rats.
Materials and Methods: In this study, 40 male Wistar rats were randomly divided into 5 groups (8 rats in each group): Control, experimental left varicocele (ELV), ELV + ethanol, ELV + 20 mg/kg RES and ELV + 50 mg/kg RES. Varicocele was induced by partial ligation of the left renal vein. Three months after varicocele induction, RESwas orally administered to rats for 1 month. The expression levels of NLRP3, apoptosis associated speck-like protein (ASC), caspase-1, Bax and Bcl2 were analyzed using real time PCR.
Results: Our results showed that RESat both doses significantly (P≤ 0.05) decreased the gene expression levels of ASC, NLRP3, caspase-1 and Bax and increased Bcl2 gene expression at high dose.
Conclusions: RESby reducing inflammatory factors and decreasing apoptosis might be used as adjuvant therapy to reduce varicocele complication.

Keywords

Main Subjects


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