An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue

Document Type: Original Article

Authors

1 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey

2 Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey

3 Department of Hematology, Baskent University, Adana, Turkey

4 Faculty of Life and Natural Sciences, Abdullah Gul University, Kayseri, Turkey

5 Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, Turkey

Abstract

Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.
Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.
Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.
Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.

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Main Subjects


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