Comparative virulotyping and phylogenomics of Escherichia coli isolates from urine samples of men and women suffering urinary tract infections

Document Type: Short Communication

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

2 Graduated student (DVM), Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

Abstract

Objective(s): Escherichia coli strains are common pathogens that can cause urinary tract infections (UTI). This study aimed to assess E. coli phylogroups and virulence types in male and female UTI patients.
Materials and Methods: In the present study, 160 uropathogenic E. coli (UPEC) isolates (from both sexes) were assigned to phylogroups/types and some extraintestinal virulence factors were detected within them by multiplex-PCR.
Results: The isolates from women and men were predominantly distributed within phylogroup B2 and D, respectively. The presence of D2 phylotype was higher in men isolates than women, significantly (P=0.045). In male isolates papEF and sfa/focDE are more prevalent in B2 group than D, significantly (P=0.048; P=0.035). The prevalence of hly in B2 group is significantly higher than D (P=0.034) in female isolates.
Conclusion: This study highlighted different features of E. coli genotypes from phylogenetic and virulence point of view implicated in UTI’s in both human genders.

Keywords

Main Subjects


1. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123-140.
2. Fratamico PM, DebRoy C, Liu Y, Needleman DS, Baranzoni GM, Feng P. Advances in molecular serotyping and subtyping of Escherichia coli. Front Microbiol 2016;7:1-10.
3. Hryniewicz K, Szczypa K, Sulikowska A, Jankowski K, Betlejewska K, Hryniewicz W. Antibiotic susceptibility of bacterial strains isolated from urinary tract infections in Poland. J Antimicrob Chemother 2001;47:773-780.
4. Farajnia S, Alikhani MY, Ghotaslou R, Naghili B, Nakhlband A. Causative agents and antimicrobial susceptibilities of urinary tract infections in the northwest of Iran. Int J Infect Dis 2009;13:140-144.
5. Carlos C, Pires MM, Stoppe NC, Hachich EM, Sato MI, Gomes TA, et al. Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination. BMC Microbiol 2010;10:161-171.
6. Salehi TZ, Tonelli A, Mazza A, Staji H, Badagliacca P, Tamai IA, et al. Genetic characterization of Escherichia coli O157: H7 strains isolated from the one-humped camel (Camelus dromedarius) by using microarray DNA technology. Mol Biotech 2012;51:283-288.
7. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2000;66:4555-4558.
8. Staji H, Khoshgoftar J, Vayeghan AJ, Bejestani MRS. Phylogenetic grouping and assessment of virulence genotypes, with antibiotic resistance patterns, of Escherichia coli strains implicated in female urinary tract infections. Int J Enteric Pathog 2016;4:16-22.
9. John AS, Mboto CI, Agbo B. A review on the prevalence and predisposing factors responsible for urinary tract infection among adults. Euro J Exp Bio 2016;6:7-11.
10.    Tandan M, Duane S, Cormican M, Murphy AW, Vellinga A. Reconsultation and antimicrobial treatment of urinary tract infection in male and female patients in general practice. Antibiotics 2016;5:31-42.
11.    Cobo G, Hecking M, Port FK, Exner I, Lindholm B, Stenvinkel P, et al. Sex and gender differences in chronic kidney disease: progression to end-stage renal disease and haemodialysis. Clin Sci 2016;130:1147-1163.
12.    Lee DS, Choe H-S, Kim HY, Yoo JM, Bae WJ, Cho YH, et al. Role of age and sex in determining antibiotic resistance in febrile urinary tract infections. Int J Infect Dis 2016;51:89-96.
13.    Bonadio M, Meini M, Spitaleri P, Gigli C. Current microbiological and clinical aspects of urinary tract infections. Eur Urol 2001;40:439-445.
14.    Staji H, Shahaboddin E, Kafshdouzan K. Correlation of Escherichia coli strains isolated from wild bird feces and human urinary tract infections from phylogenetic point of view. Avicenna J Clin Microbiol Infect 2017;4:16-25.
15.    Staji H. Detection of enterohemorrhagic Escherichia coli related genes in Escherichia coli strains belonging to B2 phylogroup isolated from urinary tract infections in combination with antimicrobial resistance phenotypes. J Med Bacteriol 2017;6:36-44.
16. Adib N, Ghanbarpour R, Solatzadeh H, Alizade H. Antibiotic resistance profile and virulence genes of uropathogenic Escherichia coli isolates in relation to phylogeny. Trop Biomed 2014;31:17-25.
17.    Bingen E, Picard B, Brahimi N, Mathy S, Desjardins P, Elion J, et al. Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. J Infect Dis 1998;177:642-650.
18.    Pupo GM, Karaolis D, Lan R, Reeves PR. Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies. Infect Immun 1997;65:2685-2692.
19.    Lee CCY. Genotyping Escherichia coli isolates from duck, goose, and gull fecal samples with phylogenetic markers using multiplex polymerase chain reaction for application in microbial source tracking. J Exp Microbiol Immun 2011;15:130-135.
20.    Blum G, Falbo V, Caprioli A, Hacker J. Gene clusters encoding the cytotoxic necrotizing factor type 1, Prs-fimbriae and α-hemolysin form the pathogenicity island II of the uropathogenic Escherichia coli strain J96. FEMS Microbiol Lett 1995;126:189-195.
21.    Johnson JR, Kuskowski MA, Owens K, Gajewski A, Winokur PL. Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J Infect Dis 2003;188:759-768.
22.    Zhang L, Foxman B, Marrs C. Both urinary and rectal Escherichia coli isolates are dominated by strains of phylogenetic group B2. J Clin Microbiol 2002;40:3951-3955.
23.    Wurpel DJ, Totsika M, Allsopp LP, Webb RI, Moriel DG, Schembri MA. Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells. J Proteom 2016;131:177-189.
24. Bien J, Sokolova O, Bozko P. Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. Int J Nephrol 2012; 2012:681473.
25. Gao Q, Wang X, Xu H, Xu Y, Ling J, Zhang D, et al. Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model. BMC Microbiol 2012;12:143-152.