Human leukocyte antigen (HLA)-Cw0303, HLA-Cw04, and HLA-Cw07 polymorphisms are associated with susceptibility of rheumatoid arthritis in Chinese Han patients from Southern China

Document Type : Original Article

Authors

Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China

Abstract

Objective(s): This study aimed to investigate the association between human leukocyte antigen Cw (HLA-Cw) polymorphisms and rheumatoid arthritis (RA) in Chinese Han patients in the Jiangsu area (Southern China).
Materials and Methods: Polymerase chain reaction-sequence specific primers were used to detect HLA-Cw01–08 of 201 RA patients and 211 healthy individuals from Zhongda Hospital (China). The allele frequency distribution of HLA-Cw and genotypic differences between the two groups were analyzed.
Results: The frequency of HLA-Cw0303 in patients with RA was significantly higher than that in controls, while the frequency of HLA-Cw04 was lower than that in controls (P<0.05). The gene frequency of HLA-Cw07 in anti-cyclic citrullinated peptide (anti-CCP)-negative patients was higher than that in controls (P=0.044). The frequency of HLA-Cw04 was decreased in the short duration subgroup and increased in the long duration subgroup (P<0.05). Compared to controls, the frequency of HLA-Cw0303 in patients with RA and morning stiffness was increased (P=0.004), while the frequency of HLA-Cw04 was decreased ( 0.005).
Conclusion: These results suggest that HLA-Cw0303 is a susceptibility gene for RA in Chinese Han patients in the Jiangsu area of southern China. The HLA-Cw04 gene may be a protective factor against RA, while HLA-Cw07 might play a protective role in the production of anti-CCP in the long-term course in patients with RA.

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1.Kelley W, Firestein G, Budd R, Gabriel S, McInnes I, I'Dell J. Kelley's textbook of rheumatology. Dublin: Elsevier Health Sciences; 2012.
2.Weyand CM, Goronzy JJ. Association of MHC and rheumatoid arthritis. HLA polymorphisms in phenotypic variants of rheumatoid arthritis. Arthritis Res 2000; 2:212-216.
3.Moretta A, Bottino C, Mingari MC, Biassoni R, Moretta L. What is a natural killer cell? Nature Immunol 2002; 3:6-8.
4.Yen JH, Moore BE, Nakajima T, Scholl D, Schaid DJ, Weyand CM, et al. Major histocompatibility complex class I-recognizing receptors are disease risk genes in rheumatoid arthritis. J Exp Med 2001; 193:1159-1167.
5.Guerini FR, Fusco C, Mazzi B, Favoino B, Nocera G, Agliardi C, et al. HLA-Cw allele frequencies in northern and southern Italy. Transpl Immunol 2008; 18:286-2896.
6.Wiśniewski A, Jankowska R, Passowiczmuszyńska E, Wiśniewska E, Majorczyk E, Nowak I, et al. KIR2DL2/S2 and HLA-C C1C1 genotype is associated with better response to treatment and prolonged survival of patients with non-small cell lung cancer in a Polish Caucasian population. Human Immunol 2012; 73:927-931.
7.Chazara O, Xiong S, Moffett A. Maternal KIR and fetal HLA-C: a fine balance. J Leukoc Biol 2011; 90:703-716.
8.Deng Z, Wang D, Xu Y, Gao S, Zhou H, Yu Q, et al. HLA-C polymorphisms and PCR dropout in exons 2 and 3 of the Cw*0706 allele in sequence-based typing for unrelated Chinese marrow donors. Human Immunol 2010; 71:577-581.
9.Gladman DD, Kung TN, Siannis F, Pellett F, Farewell VT, Lee P. HLA markers for susceptibility and expression in scleroderma. J Rheumatol 2005; 32:1481-1487.
10.Jiao YL, Ma CY, Wang LC, Cui B, Zhang J, You L, et al. Polymorphisms of KIRs gene and HLA-C alleles in patients with ankylosing spondylitis: possible association with susceptibility to the disease. J Clin Immunol 2008; 28:343-349.
11.Hou Y, Zhang C, Xu D, Sun H. Association of KIR and HLA-Cw genes combinations with systemic lupus erythematosus. Clin Exp Immunol 2015; 180:250-254.
12.Sakata Y, Kokabu S, Nojima J, Nakamoto N, Fukushima Y, Sato T, et al. Significant association of HLA-Cw and HLA-DRB1 alleles with recurrent aphthous stomatitis. J Oral Maxillofac Surg 2011; 23:7-9.
13.AlHeresh AM, Proctor J, Jones SM, Dixey J, Cox B, Welsh K, et al. Tumour necrosis factor α polymorphism and the HLA‐Cw*0602 allele in psoriatic arthritis. Rheumatol 2002; 41:525-530.
14.Zhang Y, Zhang H, Huang Y, Sun R, Liu R, Wei J. Human leukocyte antigen (HLA)-C polymorphisms are associated with a decreased risk of rheumatoid arthritis. Mol Biol Rep 2014; 41:4103-4108.
15.Mitsunaga S, Suzuki Y, Kuwana M, Sato S, Kaneko Y, Homma Y, et al. Associations between six classical HLA loci and rheumatoid arthritis: a comprehensive analysis. Tissue Antigens 2012; 80:16-25.
16.Aletaha D, Neogi T, Silman AJ, Funovits J, Felson DT, Bingham CO, et al. 2010 rheumatoid arthritis classification criteria: an american college of rheumatology/european league against rheumatism collaborative initiative. Arthritis Rheumatol 2016; 62:2569-2581.
17.Welsh K, Bunce M. Molecular typing for the MHC with PCR-SSP. Rev Immunogenet 1999; 1:157-176.
18.Fox RI, Saito I. Criteria for diagnosis of Sjogren's syndrome. Rheum Dis Clin North Am 1994; 20:391-407.
19.Jie HB, Sarvetnick N. The role of NK cells and NK cell receptors in autoimmune disease. Autoimmunity 2004; 37:147-153.
20.Li LI, Cui Y, Cheng-Taoa HE, Miao FQ. Genetic polymorphism of HLA-Cw as KIR ligands in Jiangsu Han population. Chin J Clin Lab Sci 2012.
21.He J, Chen Z, Hou J, Wu D, Bao X, Li Y, et al. Investigation on high resolution matching of HLA-Cw and effect on unrelated blood stem cen transplantation in acute leukemia. Chin J Microbiol Immunol 2007:643-646.
22.Yin X, Xiao L, Guo K, Ma H, Deng L, Ye X, et al. Expression of HLA--Cw and its corresponding stimulatory and inhibitory KIR in 79 cases of guangdong han population. Chin J Immunol 2005; 21:605-607.
23.Lavant EH, Agardh DJ, Nilsson A, Carlson JA. A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease. Clin Chim Acta 2011; 412:782-784.
24.Parham P. Immunogenetics of killer-cell immunoglobulin-like receptors. Mol Immunol 2005; 42:459-462.
25.Middleton D, Meenagh A, Wright GD. No association in frequency of KIR receptors in patients with rheumatoid arthritis from northern ireland. Tissue Antigens 2007; 69:577-582.
26.Fan-Bin LV, Zhang Y, Xia P. Influence of HLA-Cw immuno regulation on the survival of renal transplant recipients. Chin J Transpl 2012.
27.Zeng JQ, Xu YP, Wang DM, Gao SQ, Zou HY, Deng ZH. Single nucleotide polymorphisms in 22 HLA-Cw alleles in chinese han population. Hereditas 2010; 32:473-479.
28.Sun H, Li LI, Fan W, Shi Y, Chengtao HE, Cui Y. Investigation of the killer cell immunoglobulin-like receptors gene polymorphisms of patients with rheumatoid arthritis. Journal of Molecular Diagnostics & Therapy. 2010.
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30.Stewart CA, Laugier-Anfossi F, Vély F, Saulquin X, Riedmuller J, Tisserant A, et al. Recognition of peptide-MHC class I complexes by activating killer immunoglobulin-like receptors. Proc Natl Acad Sci U S A  2005; 102:13224-13229.
31.Jiang Z, Xiang-Pei LI, Qian L, Zhang H. Influences of HLA-DRB1 alleles and anti-CCP antibody on the arthrosis erosion of rheumatoid arthritis. Anhui Med Pharm J 2011; 5.
32.Li HB, Tie N, Jia YF, Shi L, Su Y, Zhang GZ, et al. Association between the synovial expression of cyclic citrullinated peptide and susceptibility variants of HLA-DRB1 shared epitope alleles and PADI 4 gene single nucleotide polymorphisms in patients with rheumatoid arthritis. Zhonghua Yi Xue Za Zhi 2012; 92:1607-1611.
33.de Vries RR, Huizinga TW, Toes RE. Redefining the HLA and RA association: to be or not to be anti-CCP positive. J Autoimmun 2005; 25:21-25.