The protective effect of Liza klunzingeri protein hydrolysate on carbon tetrachloride-induced oxidative stress and toxicity in male rats

Document Type: Original Article

Authors

1 Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran

2 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Department of Pathobiology and Quality Control, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran

4 Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Objective(s): Today, consumers are looking for food products providing health benefits in addition to meeting the basic nutritional needs of the body. This study aimed to evaluate the antioxidant and cytotoxic effects of Liza klunzingeri protein hydrolysate both in vivo and in vitro.
Materials and Methods: Fish protein hydrolysate (FPH) was prepared using enzymatic hydrolysis with papain. In vitro antioxidant activity was assessed using five different antioxidant assays. The cytotoxic effect on 4T1 cell line was evaluated using the MTT assay. The distribution of the molecular weight of FPH was measured using HPLC. In the in vivo study, CCl4-exposed Wistar rats were orally treated with FPH (150, 300, and 600 mg/kg) or gallic acid (50 mg/kg) for 28 consecutive days.
Results: Enzymatic hydrolysis gave hydrolysate rich in low molecular weight peptides (Conclusion: L. klunzingeri protein hydrolysate can be considered as a functional food to alleviate oxidative stress.

Keywords

Main Subjects


1. Farzamirad V, Aluko RE. Angiotensin-converting enzyme inhibition and free-radical scavenging properties of cationic peptides derived from soybean protein hydrolysates. Int J Food Sci Nutr 2008; 59:428-437.
2. Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sánchez-Pérez P, Cadenas S, Lamas S. Antioxidant responses and cellular adjustments to oxidative stress. Redox Biol 2015; 6: 183-197.
3. Nazeer R, Anila Kulandai K. Evaluation of antioxidant activity of muscle and skin protein hydrolysates from giant kingfish, Caranx ignobilis (Forsskål, 1775). Int J Food Sci Tech 2012; 47:274-281.
4. Zhao L, Luo Y-C, Wang C-T, Ji B-P. Antioxidant activity of protein hydrolysates from aqueous extract of velvet antler (Cervus elaphus) as influenced by molecular weight and enzymes. Nat Prod Commun 2011; 6:1683-1688.
5. Khan MR, Rizvi W, Khan GN, Khan RA, Shaheen S. Carbon tetrachloride-induced nephrotoxicity in rats: Protective role of Digera muricata. J Ethnopharmacol 2009; 122:91-99.
6. Sreelatha S, Padma P, Umadevi M. Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats. Food Chem Toxicol 2009; 47:702–708.
7. Srivastava A, Shivanandappa T. Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats. Food Chem 2010; 118:411-417.
8. Zhang H, Tsao R. Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects. Curr Opin Food Sci 2016; 8:33-42.
9. Giampieri F, Alvarez-Suarez JM, Mazzoni L, Forbes-Hernandez TY, Gasparrini M, Gonzalez-Paramas AM, et al. An anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial functionality in human dermal fibroblasts exposed to an oxidizing agent. Food Funct 2014; 5:1939-1948.
10. Tokudome Y, Nakamura K, Kage M, Todo H, Sugibayashi K, Hashimoto F. Effects of soybean peptide and collagen peptide on collagen synthesis in normal human dermal fibroblasts. Int J Food Sci Nutr 2012; 63:689-695.
11. Dziuba M, Darewicz M. Food proteins as precursors of bioactive peptides—classification into families. Food Sci Technol Int 2007; 13:393-404.
12. Park E, Paik H-D, Lee S-M. Combined effects of whey protein hydrolysates and probiotics on oxidative stress induced by an iron-overloaded diet in rats. Food Sci Nutr 2017:1-10.
13. Tang W, Zhang H, Wang L, Qian H, Qi X. Targeted separation of antibacterial peptide from protein hydrolysate of anchovy cooking wastewater by equilibrium dialysis. Food Chem 2015; 168:115-123.
14. Prabhu S, Dennison SR, Lea B, Snape TJ, Nicholl ID, Radecka I, et al. Anionic antimicrobial and anticancer peptides from plants. CRC Crit Rev Plant Sci 2013; 32:303-320.
15. Agyei D, Danquah MK. Rethinking food-derived bioactive peptides for antimicrobial and immunomodulatory activities. Trends Food Sci Technol 2012; 23:62-69.
16. Athmani N, Dehiba F, Allaoui A, Barkia A, Bougatef A, Lamri-Senhadji MY, et al. Sardina pilchardus and Sardinella aurita protein hydrolysates reduce cholesterolemia and oxidative stress in rat fed high cholesterol diet. J Exp Integr Med 2015; 5:47.
17. Murray B, FitzGerald R. Angiotensin converting enzyme inhibitory peptides derived from food proteins: biochemistry, bioactivity and production. Curr Pharm Des 2007; 13:773-791.
18. Kim S-K, Mendis E. Bioactive compounds from marine processing byproducts–a review. Food Res Int 2006; 39:383-393.
19. FAO. Fishery and Aquaculture Statistics 2016. Rome, Italy: 2016.
20. Hakimelahi M, Kamrani E, Taghavi Motlagh S, Ghodrati Shojaei M, Vahabnezhad A. Growth parameters and mortality rates of Liza klunzingeri in the Iranian waters of the Persian Gulf and Oman Sea, using Length Frequency Data. Iranian J Fish Sci 2010; 9:87-96.
21. AOAC. Methods of analysis. Washington DC; AOAC: 1989.
22. Nikoo M, Benjakul S, Xu X. Antioxidant and cryoprotective effects of Amur sturgeon skin gelatin hydrolysate in unwashed fish mince. Food Chem 2015; 181:295-303.
23. Guo L, Hou H, Li B, Zhang Z, Wang S, Zhao X. Preparation, isolation and identification of iron-chelating peptides derived from Alaska pollock skin. Process Biochem 2013; 48:988-993.
24. Namıduru E, Tarakçoğlu M, Namıduru M, Kocabaş R, Erbağcı B, Meram I, et al. Increased serum nitric oxide and malondialdehyde levels in patients with acute intestinal amebiasis. Asian Pac J Trop Biomed 2011; 1:478-481.
25. Wong FW-Y, Chan W-Y, Lee SS-T. Resistance to carbon tetrachloride-induced hepatotoxicity in mice which lack CYP2E1 expression. Toxicol Appl Pharmacol 1998; 153:109-118.
26. Khaled HB, Ghlissi Z, Chtourou Y, Hakim A, Ktari N, Fatma MA, et al. Effect of protein hydrolysates from sardinelle (Sardinella aurita) on the oxidative status and blood lipid profile of cholesterol-fed rats. Food Res Int 2012; 45:60-68.
27. Ktari N, Nasri R, Mnafgui K, Hamden K, Belguith O, Boudaouara T, et al. Antioxidative and ACE inhibitory activities of protein hydrolysates from zebra blenny (Salaria basilisca) in alloxan-induced diabetic rats. Process Biochem 2014; 49:890-897.
28. Sarmadi BH, Ismail A. Antioxidative peptides from food proteins: a review. Peptides 2010; 31:1949-1956.
29. Erdmann K, Grosser N, Schipporeit K, Schröder H. The ACE inhibitory dipeptide Met-Tyr diminishes free radical formation in human endothelial cells via induction of heme oxygenase-1 and ferritin. J Nutr 2006; 136:2148-2152.
30. Ktari N, Mnafgui K, Nasri R, Hamden K, Bkhairia I, Hadj AB, et al. Hypoglycemic and hypolipidemic effects of protein hydrolysates from zebra blenny (Salaria basilisca) in alloxan-induced diabetic rats. Food Funct 2013; 4:1691-1699.
31. Rabiei S, Nikoo M, Rezaei M, Rafieian-Kopaei M. Marine-Derived Bioactive Peptides with Pharmacological Activities-A Review. J Clin Diagn Res 2017; 11.
32.Harnedy PA, FitzGerald RJ. Bioactive peptides from marine processing waste and shellfish: A review. J Funct Foods 2012; 4:6-24.
33. Grimble G, Keohane P, Higgins B, Kaminski M, Silk D. Effect of peptide chain length on amino acid and nitrogen absorption from two lactalbumin hydrolysates in the normal human jejunum. Clin Sci 1986; 71:65-69.