Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats

Document Type: Original Article

Authors

1 Department of Internal Medicine, Kayseri City Hospital, Kayseri, Turkey

2 Department of Nephrology, Erciyes University Medical Faculty, Kayseri, Turkey

3 Department of Medical Biochemistry, Ömer Halisdemir University School of Medicine, Niğde, Turkey

4 Department of Pathology, Erciyes University Medical Faculty, Kayseri, Turkey

5 Department of Medical Genetics, Erciyes University Medical Faculty, Kayseri, Turkey

6 Department of Urology, Kayseri City Hospital, Kayseri, Turkey

7 Department of Biostatistics, İzmir Katip Çelebi Üniversitesi Medical Faculty, İzmir, Turkey

8 Department of Biochemistry, Erciyes University Medical Faculty, Kayseri, Turkey

Abstract

Objective(s): This study aimed to show the effects of thymoquinone, which is known for its antioxidant, anti-inflammatory, and renal protective effects in contrast-induced nephropathy.
Materials and Methods: This is an experimental study in rats. 7 groups were included within the scope of our study: sham-vehicle (n=3), premedication-control (n=6), model (n=6), isolated thymoquinone (n=3+3), low-dose thymoquinone (n=6), and high-dose thymoquinone (n=7). In addition to 48 hr of water deprivation, we pre-medicated the rats with intra-peritoneal indomethacin and L-NAME administration. After premedication, 12.5 ml/kg dose of a high osmolar contrast agent-diatrizoat (Urografin %76) was administrated. Thymoquinone was administrated in two different doses of 1 mg/kg and 1.75 mg/kg for four days intraperitoneally. Renal functions, histopathological differences, oxidative stress parameters, and inflammatory indicators of rats were evaluated at the end of the study.
Results: Significant decreases were observed in levels of serum creatinine and serum BUN with low-dose thymoquinone (1 mg/kg) administration. In light microscopy, significantly less histopathological damage was observed in the low-dose thymoquinone group compared to the contrast agent group. While high-dose thymoquinone is accepted as ineffective biochemically, toxic evidence was identified histopathologically. There were no significant differences between M and TA groups for serum MDA and SOD levels, which were compared to evaluate oxidative stress (P:0.99, P:0.98; respectively). TNF-α, iNOS, and NF-кB gene expressions were not significantly different between all groups (P:0.748, P:0.531, P:0.910; respectively).
Conclusion: This experimental study has demonstrated for the first time the protective effect of the TQ substance for CIN in 1 mg/kg dose, in the accompaniment of biochemical and histopathological data in rats.

Keywords


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