Study the Antinociceptive Effect of Intracerebroventricular Injection of Aqueous Extract of Origanum Vulgare Leaves in Rat: Possible Involvement of Opioid System

Authors

1 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

2 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran 2 Department of Physiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Departments of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

4 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

5 Faculty of Medicine, Ardabil Azad University of Medical Sciences, Ardabil, Iran

Abstract

 

Objective(s):
The aim of study was to investigate the antinociceptive effect of intracerebroventricular (ICV) microinjection of Origanum vulgare (ORG) extract and possible involvement of opioid receptors.
 
Materials and Methods:
Cannula was inserted into left ventricle of male rats. Five days after surgery Tail Flick Latency (TFL) was measured after ICV microinjection of, ORG (1, 3 and 6 μg / rat). Effective dose of ORG was injected ICV in concomitant with morphine (2 mg/kg, IP), naloxone (2 mg / kg, IP) and saline (0.5 μl/rat) and TFL was recorded.
Results:
The co- administration of ORG extract with morphine showed a significant increase in TFL and naloxone, pretreatment significantly inhibited the antinociceptive activity of ORG and morphine.
Conclusion:
The aqueous extract of ORG possesses antinociceptive activities in the tail-flick test in a dose dependent manner. ORG - induced antinociception may have been mediated by opioid systems.

Keywords


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Pahlavan et al OriganumVulgare Antinociception
Iran J Basic Med Sci, Vol. 16, No. 10, Oct 2013
 
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