Study the Antinociceptive Effect of Intracerebroventricular Injection of Aqueous Extract of Origanum Vulgare Leaves in Rat: Possible Involvement of Opioid System

Authors

1 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

2 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran 2 Department of Physiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Departments of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

4 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

5 Faculty of Medicine, Ardabil Azad University of Medical Sciences, Ardabil, Iran

Abstract

 

Objective(s):
The aim of study was to investigate the antinociceptive effect of intracerebroventricular (ICV) microinjection of Origanum vulgare (ORG) extract and possible involvement of opioid receptors.
 
Materials and Methods:
Cannula was inserted into left ventricle of male rats. Five days after surgery Tail Flick Latency (TFL) was measured after ICV microinjection of, ORG (1, 3 and 6 μg / rat). Effective dose of ORG was injected ICV in concomitant with morphine (2 mg/kg, IP), naloxone (2 mg / kg, IP) and saline (0.5 μl/rat) and TFL was recorded.
Results:
The co- administration of ORG extract with morphine showed a significant increase in TFL and naloxone, pretreatment significantly inhibited the antinociceptive activity of ORG and morphine.
Conclusion:
The aqueous extract of ORG possesses antinociceptive activities in the tail-flick test in a dose dependent manner. ORG - induced antinociception may have been mediated by opioid systems.

Keywords


1. Mockute D, Bernotiene G, Judzentiene A. The essential oil of Origanum vulgare L. ssp. vulgare growing wild in vilnius district (Lithuania). Phytochemistry 2001; 57:65

.

2. Pande C, Tewari G, Singh S, Singh C. Chemical markers in Origanum vulgare L. from Kumaon Himalayas: a chemosystematic study. Nat Prod Res 2012; 26:140-415

.

3. Srihari T, Sengottuvelan M, Nalini N. Dose-dependent effect of oregano (Origanum vulgare L.) on lipid peroxidation and antioxidant status in 1,2-dimethylhydrazine-induced rat colon carcinogenesis. J Pharm Pharmacol 2008; 60:787-794.

4. Vale-Silva L, Silva MJ, Oliveira D, Gonçalves MJ, Cavaleiro C, Salgueiro L, et al. Chemical constituents and biological studies of Origanum vulgare Linn. J Med Microbiol 2012; 61:252.

5. Hernández-Hernández E, Ponce-Alquicira E, Jaramillo-Flores ME, Guerrero Legarreta I. Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Sci 2009; 81:410-147.

6. De Martino L, De Feo V, Formisano C, Mignola E, Senatore F. Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart growing wild in Campania (Southern Italy). Molecules 2009; 14: 2735-7746.

7. Karakaya S, El SN, Karagözlü N, Sahin S. Antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) by using different extraction methods. J Med Food 2011; 14:645-652.

8. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 4th ed. San Diego: Academic.Press; 1998.p. 467.

9. Sepehri G , Sheibani V , Azarang A, Shamsizadeh A, Afarinesh MR, Azizollahi S, et al. The intracerebroventricular (ICV) administration of W-7, a calmodulin inhibitor, attenuates the development of morphine tolerance in rats. Pak J Pharm Sci 2010; 23:170-174.

10. Guimarães AG, Oliveira GF, Melo MS, Cavalcanti SC, Antoniolli AR, Bonjardim LR, et al. Bioassay guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic Clin Pharmacol Toxicol 2010; 107:949-957.

11. Mikaili P, Mohammad Nezhady MA, Shayegh J, Asghari MH. Study of antinociceptive effect of Nepeta meyeri, Raphanus sativus and Origanum vulgare extracts in mouse by acute pain assessment method. Int J Acad Res 2010; 2:1-3.

12. Arzi A, Aghel N, Nazari Khorasgani Z, Motahari M. The study of analgesic effect of hydroalcoholic extract of Origanum vulgare in rat by formalin test. Toxicol Lett 2009; 189:105.

13. Gorji A. Pharmacological treatment of headache using traditional Persian medicine. Trends Pharmacol Sci 2003; 24:331-334.

Pahlavan et al OriganumVulgare Antinociception

Iran J Basic Med Sci, Vol. 16, No. 10, Oct 2013

 

1 1 1 3

14. Kazemi M, Rostami H , Ameri A.The study of compositions and antimicrobial properties of essential oil of Origanum vulgare and rosmarinus officinalis on human pathogens. . Curr Res Bacteriol 2012; 5:1-12.

15. Melo FHC, Rios ERV, Rocha NFM, Citó MCO, Fernandes ML, Sousa DP, et al. Antinociceptive activity of carvacrol (5-isopropyl-2- methylphenol) in mice. J Pharm Pharmocol 2012; 64:1722–1729.