Effects of chronic crocin treatment on desoxycorticosterone acetate (doca)-salt hypertensive rats

Document Type: Original Article

Authors

1 Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

2 Targeted Drug Delivery Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

3 School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

 




Objective(s):
In this study, the effects of chronic administration of crocin, an active constituent of saffron, on blood pressures of normotensive and desoxycorticosterone acetate (DOCA) - salt induced hypertensive rats, were investigated.
 
Materials and Methods:
Five week administration of three doses of crocin (50, 100 and 200 mg/kg/day) and spironolactone (50 mg/kg/day) in different groups of normotensive and hypertensive rats (at the end of 4 weeks treatment by DOCA-salt) was carried out and their effects on mean systolic blood pressure (MSBP) and heart rate (HR) were evaluated using tail cuff method. The duration of effect of crocin on SBP, was also evaluated.
Results:
Our results indicated that chronic administration of crocin could reduce the MSBP in DOCA salt treated rats in a dose dependent manner. Crocin did not decrease the MSBP in normotensive rats. The data also showed that antihypertensive effects of crocin did not persist.
Conclusion:
It is concluded that crocin possesses antihypertensive and normalizing effect on BP in chronic administration.

Keywords


 

1. Ríos J, Recio M, Giner R, Manez S. An update review of saffron and its active constituents. Phytother Res 1996; 10:189-193.

2. Fernández J. Anticancer properties of saffron,

Crocus sativus Linn. Adv Phytomed 2006; 313-330.

3. Hosseinzadeh H, Behravan J, Ramezani M, Ajgan Kh. Anti-tumor and cytotoxic evaluation of

Crocus sativus L. stigma and petal extracts using brine shrimp and potato disc assays. J Med Plants 2005; 4:59-65.

4. Hosseinzadeh H, Shamsaie F, Mehri S. Antioxidant activity of aqueous and ethanolic extracts of

Crocus sativus L. stigma and its bioactive constituents crocin and safranal. Pharmacogn Mag 2010; 5:419-424.

5. Sheng L, Qian Z, Zheng S, Xi L. Mechanism of hypolipidemic effect of crocin in rats: crocin inhibits pancreatic lipase. Eur J Pharmacol 2006; 543:116-122.

6. Amin B, Hosseinzadeh H. Evaluation of aqueous and ethanolic extracts of saffron,

Crocus sativus L., and its constituents, safranal and crocin in allodynia and hyperalgesia induced by chronic constriction injury model of neuropathic pain in rats. Fitoterapia 2012; 83:888-895.

7. Vosooghi S, Mahmoudabady M, Neamati A, Aghababa H. Crocin alleviates the local paw edema induced by histamine in rats. Avicenna J Phytomed 2012; 2:97-104.

8. Hosseinzadeh H, Talebzadeh F. Anticonvulsant evaluation of safranal and crocin from

Crocus sativus in mice. Fitoterapia 2005; 76:722-724.

9. Hosseinzadeh H, Karimi G, Niapoor M. Antidepressant effects of

Crocus sativus stigma extracts and its constituents, crocin and safranal, in mice. J Med Plants 2004; 3:48-58.

10. Hosseinzadeh H, Sadeghnia HR, Ghaeni FA, Motamedshariaty VS, Mohajeri SA. Effects of saffron (

Crocus sativus L.) and its active constituent, crocin, on recognition and spatial memory after chronic cerebral hypoperfusion in rats. Phytother Res 2012; 26:381-386.

11. Hosseinzadeh H, Ziaei T. Effects of

Crocus sativus stigma extract and its constituents, crocin and safranal, on intact memory and scopolamine-induced learning deficits in rats performing the Morris water maze task. J Med Plants 2006; 5:40-50.

12. Razavi M, Hosseinzadeh H, Abnous K, Motamedshariaty V, Imenshahidi M. Crocin restores hypotensive effect of subchronic administration of diazinon in rats. Iran J Basic Med Sci 2013; 16:64-72.

13. Hariri A, Moallem S, Mahmoudi M, Hosseinzadeh H. The effect of crocin and safranal, constituents of saffron, against subacute effect of diazinon on hematological and genotoxicity indices in rats. Phytomedicine 2011; 18

:499-504.

14. Mehri S, Abnous K, Mousavi S, Motamed Shariaty V, Hosseinzadeh H. Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells. Cell Mol Neurobiol 2012; 32:227-235.

15. Boskabady MH, Shafei MN, Shakiba A, Sang Sefidi H. Effect of aqueous-ethanol extract from Crocus sativus (saffron) on guinea-pig isolated heart. Phytother Res 2008; 22:330-334.

16. Fatehi M, Rashidabady T, Hassanabad ZF. Effects of petals extracts of saffron on rat blood pressure and on responses induced by electrical field stimulation in the rat isolated vas deferens and guinea–pigileum. J Ethnopharmacol 2003; 84:199–203.

17. Imenshahidi M, Hosseinzadeh H, Javadpour Y. Hypotensive effect of aqueous saffron extract (

Crocus sativus L.) and its constituents, safranal and crocin, in normotensive and hypertensive rats. Phytother Res 2010; 24:990–994.

18. Hadizadeh F, Mohajeri S, Seifi M. Extraction and purification of crocin from saffron stigmas employing a simple and efficient crystallization method. Pak J Biol Sci 2010; 13:691-698.

19. Lorenz JN. A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice. Am J Physiol Regul Integr Comp Physiol 2002; 282:R1565-1582.

20. Boskabadi MH, Aslani MR. Relaxant effect of

crocus sativus (saffron) on guinea pig tracheal chains and its possible mechanisms. J Pharm Pharmacol 2006; 58:1385-1390.

Imenshahidi et al Effect of crocin chronic administration on blood pressure

Iran J Basic Med Sci, Vol. 17, No. 1, Jan 2014 13

 

21. He S, Qian Z, Tang F. Effect of crocin on intracellular calcium concentration in cultured bovine aortic smooth muscle cells. Acta Pharm Sin 2004; 39:778-781.

22. Williams B, Liu C, Deyoung L, Brock G, Sims S.Regulation of intracellular Ca2+ release in corpus cavernosum smooth muscle: synergism between nitric oxide and cGMP. Am J Physiol Cell Physiol 2005; 288:C650–C658.

23. Lyer A, Chan V, Brown L. The DOCA-Salt hypertensive rat as a model of cardiovascular oxidative and inflammatory stress. Curr Cardiol Rev 2010; 6:291-297.

24. Fatehi-Hassanabad Z, Fatehi M, Imen Shahidi M. Endothelial dysfunction in aortic rings and mesenteric beds isolated from deoxycorticosterone acetate hypertensive rats: possible involvement of protein kinase C. Eur J Pharmacol 2004; 494:199-204.

25. Iwazu Y, Muto S, Fujisawa G, Nakazawa E, Okada K, Ishibashi S,

et al. Spironolactone suppresses peritubular capillary loss and prevents deoxycorticosterone acetate/salt-induced tubulointerstitial fibrosis. Hypertension 2008; 51:749-754.

26. Tang FT, Qian ZY, Liu PQ, Zheng SG, He SY, Bao LP,

et al. Crocetin improves endothelium-dependent relaxation of thoracic aorta in hypercholesterolemic rabbit by increasing eNOS activity. Biochem Pharmacol 2006; 72:558-565.

27. Cheng S, Suzuki K, Sadee W, Harding B. Effects of spironolactone, canrenone and canrenoate-K on cytochrome P450, and 11beta- and 18-hydroxylation in bovine and human adrenal cortical mitochondria. Endocrinology 1974; 99:1097-1106.

28. Mousavi SZ, Bathaie SZ. Historical uses of saffron: Identifying potential new avenues for modern Research. Avicenna J Phytomed 2011; 1:57-66.