The effects of whole green tea infusion on mouse urinary bladder chemical carcinogenesis

Authors

1 Department of Veterinary Sciences, ECAV, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, Portugal

2 Department of Zootechnics, University of Trás-os-Montes and Alto Douro, 911 Vila Real, Portugal Center for the Study of Animal Sciences (CECA), University of Porto, 4485-661, Porto, PortugalCentre for the Research and Technology

3 Department of Veterinary Sciences, ECAV, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, PortugalCenter for the Study of Animal Sciences (CECA), University of Porto, 4485-661, Porto, PortugalCentre for the Research and Technology

4 Chemistry Research Center, CQ-VR, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal

Abstract

Objective(s):Green tea (GT) is one of the most popular beverages worldwide whose beneficial effects on health have been demonstrated. Recent studies suggest that GT may contribute to reduction of cancer risk and progression. The aim of this study was to evaluate the effects of whole GT on urinary bladder chemical carcinogenesis in male and female ICR mice.
Materials and Methods: The GT characterization was performed using spectrophotometric methods. Urinary bladder lesions were induced using N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) by gavage during 10 weeks and whole GT (0.5%) was provided ad libitum during 20 weeks.
Results:Animals from groups BBN+GT and BBN only developed preneoplastic lesions.
Conclusion:We did not observe any effects by GT infusion administration on urinary bladder cancer development.

Keywords


1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011; 61:69-90.

2. Fernandez RC, Driscoll TR, Glass DC, Vallance D, Reid A, Benke G,et al. A priority list of occupational carcinogenic agents for preventative action in Australia. Aust N Z J Public Health 2012; 36:111-115.

3. Ketabchi AA, Moshtaghi-Kashanian GR. Urinary schistosomiasis with simultaneous bladder squamous cell carcinoma and transitional cell carcinoma. Iran J Parasitol 2012; 7:96-98.

4. Palmeira C, Oliveira PA, Arantes-Rodrigues R, Colaco A, De la Cruz PLF, Lopes C,et al. DNA cytometry and kinetics of rat urothelial lesions during chemical carcinogenesis. Oncol Rep 2009; 21:247-252.

5. Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea - A review. J Am Coll Nutr 2006; 25:79-99.

6. Davari H, Haddad F, Moghimi A, Rahimi MF, Ghavamnasiri MR. Study of radioprotective effect of green tea against gamma irradiation using micronucleus assay on binucleated human lymphocytes. Iran J Basic Med Sci 2012; 15:1026-1031.

7. Lambert JD, Hong J, Yang GY, Liao J, Yang CS. Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations. Am J Clin Nutr 2005; 81:284S-291S.

8. Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M,et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 2000; 72:35-42.

9. Liebert M, Licht U, Bohm V, Bitsch R. Antioxidant properties and total phenolics content of green and black tea under different brewing conditions. Z Lebensm Unters F A 1999; 208:217-220.

10. Oliveira PA, Arantes-Rodrigues R, Sousa-Diniz C, Colaico A, Lourenco L, De la Cruz LF,et al. The effects of sirolimus on urothelial lesions chemically induced in ICR Mice by BBN. Anticancer Res 2009; 29:3221-3226.

11. Eble J, Sauter G, Epstein J, Sesterhenn I. Pathology and genetics of tumours of the urinary system and male ganital organs. WHO, IARC Press; 2004.

12. Sato D, Matsushima M. Preventive effects of urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine in rat by green tea leaves. Int J Urol 2003; 10:160-166.

13. Bianchi A, Marchetti N, Scalia S. Photodegradation of (-)-epigallocatechin-3-gallate in topical cream formulations and its photostabilization. J Pharm Biomed Anal 2011; 56:692-697.

14. Arantes-Rodrigues R, Henriques A, Pinto-Leite R, Faustino-Rocha A, Pinho-Oliveira J, Teixeira-Guedes C,

et al. The effects of repeated oral gavage on the health of male CD-1 mice. Lab Anim 2012; 41:129-134.

15. Miyamoto H, Yang Z, Chen YT, Ishiguro H, Uemura H, Kubota Y,et al. Promotion of bladder cancer development and progression by androgen receptor signals. J Natl Cancer Inst 2007; 99:558-568.