Synthesis of bone biocompatible implants using human adipose-derived mesenchymal stem cells (hADMSCs) and PCL/laminin scaffold substrate

Document Type : Original Article

Authors

1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

2 Medical Genetics Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s): Bone tissue engineering is considered a new method in the treatment of bone defects and can be an effective alternative to surgery and bone grafting. The use of adipose tissue mesenchymal stem cells (ADMSCs) on synthetic polymer scaffolds is one of the new approaches in bone tissue engineering. In this study, we aimed to investigate the effect of laminin coating on biocompatibility and osteogenic differentiation of ADMSCs seeded on polycaprolactone (PCL) scaffolds.
Materials and Methods: The morphology of the electrospun scaffold was evaluated using a scanning electron microscope (SEM). Cell proliferation and cytotoxicity were determined by MTT assay. The adipogenic and osteogenic differentiation potential of the cells was evaluated. The osteogenic differentiation of ADMSCs cultured on the PCL scaffold coated with laminin was assessed by evaluating the level of alkaline phosphatase (ALP) activity, intracellular calcium content, and expression of bone-specific genes.
Results: The results showed that the ADMSCs cultured on PCL/laminin showed enhanced osteogenic differentiation compared to those cultured on non-coated PCL or control medium (P<0.05).
Conclusion: It seems that laminin enhances the physicochemical properties and biocompatibility of PCL nanofiber scaffolds; and by modifying the surface of the scaffold, improves the differentiation of ADMSCs into osteogenic cells.

Keywords

Main Subjects

References

1. Saburi E, Islami M, Hosseinzadeh S, Moghadam AS, Mansour RN, Azadian E, et al. In vitro osteogenic differentiation potential of the human induced pluripotent stem cells augments when grown on Graphene oxide-modified nanofibers. Gene 2019; 696:72-79.
2. Hosseini FS, Soleimanifar F, Aidun A, Enderami SE, Saburi E, Marzouni HZ, et al. Poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix. J Cell Physiol 2019; 234:11537-11544.
3. Abazari MF, Soleimanifar F, Enderami SE, Nematzadeh M, Nasiri N, Nejati F, et al. Incorporated‐bFGF polycaprolactone/polyvinylidene fluoride nanocomposite scaffold promotes human induced pluripotent stem cells osteogenic differentiation. J Cell Biochem 2019; 120:16750-16759.
4. Hosseini FS, Enderami SE, Hadian A, Abazari MF, Ardeshirylajimi A, Saburi E, et al. Efficient osteogenic differentiation of the dental pulp stem cells on β‐glycerophosphate loaded polycaprolactone/polyethylene oxide blend nanofibers. J Cell Physiol 2019; 234:13951-13958.
5. Abazari MF, Hosseini Z, Karizi SZ, Norouzi S, Faskhoudi MA, Saburi E, et al. Different osteogenic differentiation potential of mesenchymal stem cells on three different polymeric substrates. Gene 2020; 740:144534.
6. Soleimanifar F, Hosseini FS, Atabati H, Behdari A, Kabiri L, Enderami SE, et al. Adipose‐derived stem cells‐conditioned medium improved osteogenic differentiation of induced pluripotent stem cells when grown on polycaprolactone nanofibers. J Cell Physiol 2019; 234:10315-10323.
7. Dobre O, Oliva MA, Ciccone G, Trujillo S, Rodrigo‐Navarro A, Venters DC, et al. A hydrogel platform that incorporates laminin isoforms for efficient presentation of growth factors–neural growth and osteogenesis. Adv Funct Mater 2021; 31:2010225.
8. Mirzaei A, Moghadam AS, Abazari MF, Nejati F, Torabinejad S, Kaabi M, et al. Comparison of osteogenic differentiation potential of induced pluripotent stem cells on 2D and 3D polyvinylidene fluoride scaffolds. J Cell Physiol 2019; 234:17854-17862.
9. Abazari MF, Soleimanifar F, Amini Faskhodi M, Mansour RN, Amini Mahabadi J, Sadeghi S, et al. Improved osteogenic differentiation of human induced pluripotent stem cells cultured on polyvinylidene fluoride/collagen/platelet‐rich plasma composite nanofibers. J Cell Physiol 2020; 235:1155-1164.
10. Abazari MF, Soleimanifar F, Amini Faskhodi M, Mansour RN, Amini Mahabadi J, Sadeghi S, et al. Improved osteogenic differentiation of human induced pluripotent stem cells cultured on polyvinylidene fluoride/collagen/platelet-rich plasma composite nanofibers. J Cell Physiol 2020; 235:1155-1164.
11. Tahmasebi A, Enderami SE, Saburi E, Islami M, Yaslianifard S, Mahabadi JA, et al. Micro‐RNA‐incorporated electrospun nanofibers improve osteogenic differentiation of human‐induced pluripotent stem cells. J Biomed Mater Res A 2020; 108:377-386.
12. Daliri Shadmehri F, Karimi E, Saburi E. Electrospun PCL/fibrin scaffold as a bone implant improved the differentiation of human adipose-derived mesenchymal stem cells into osteo-like cells. Int J Polym Mater Po 2022:1-8.  
13. Arab F, Aghaee Bakhtiari SH, Pasdar A, Saburi E. Evaluation of osteogenic induction potency of miR-27a-3p in adipose tissue-derived human mesenchymal stem cells (AD-hMSCs). Mol Biol Rep 2023; 50:1281-1291.
14. Tan F, Al-Rubeai M. Customizable implant-specific and tissue-specific extracellular matrix protein coatings fabricated using atmospheric plasma. Front Bioeng Biotechnol 2019; 7:247-262.
15. Siler U, Seiffert M, Puch S, Richards A, Torok-Storb B, Müller CA, et al. Characterization and functional analysis of laminin isoforms in human bone marrow. Blood 2000; 96:4194-4203.
16. Gu Y-C, Kortesmaa J, Tryggvason K, Persson J, Ekblom P, Jacobsen S-E, et al. Laminin isoform–specific promotion of adhesion and migration of human bone marrow progenitor cells. Blood 2003; 101:877-885.
17. Yoshikawa M, Tsuji N, Shimomura Y, Hayashi H, Ohgushi H, editors. Effects of laminin for osteogenesis in porous hydroxyapatite. Macromol Symp 2007; 253:172-178.
18. Rahmani A, Hashemi‐Najafabadi S, Eslaminejad MB, Bagheri F, Sayahpour FA. The effect of modified electrospun PCL‐nHA‐nZnO scaffolds on osteogenesis and angiogenesis. J Biomed Mater Res A 2019; 107:2040-2052.
19. Abazari MF, Zare Karizi S, Kohandani M, Nasiri N, Nejati F, Saburi E, et al. MicroRNA‐2861 and nanofibrous scaffold synergistically promote human induced pluripotent stem cells osteogenic differentiation. Polym Adv Technol 2020; 31:2259-2269.
20. Abazari MF, Hosseini Z, Zare Karizi S, Norouzi S, Amini Faskhoudi M, Saburi E, et al. Different osteogenic differentiation potential of mesenchymal stem cells on three different polymeric substrates. Gene 2020; 740:144534.
21. Abazari MF, Soleimanifar F, Enderami SE, Nematzadeh M, Nasiri N, Nejati F, et al. Incorporated-bFGF polycaprolactone/polyvinylidene fluoride nanocomposite scaffold promotes human induced pluripotent stem cells osteogenic differentiation. J Cell Biochem 2019; 120:16750-16759.
22. Ma J, Lin L, Zuo Y, Zou Q, Ren X, Li J, et al. Modification of 3D printed PCL scaffolds by PVAc and HA to enhance cytocompatibility and osteogenesis. RSC Adv 2019; 9:5338-5346.
23. Hosseini FS, Soleimanifar F, Aidun A, Enderami SE, Saburi E, Marzouni HZ, et al. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix. J Cell Physiol 2019; 234:11537-11544.
24. Jeon H, Yun S, Choi E, Kang D, Park K-h, Kim D, et al. Proliferation and osteogenic differentiation of human mesenchymal stem cells in PCL/silanated silica composite scaffolds for bone tissue regeneration. J Ind Eng Chem 2019; 79:41-51.
25. Min S-K, Kang HK, Jang DH, Jung SY, Kim OB, Min B-M, et al. Titanium surface coating with a laminin-derived functional peptide promotes bone cell adhesion. Biomed Res Int 2013; 2013:1-8.
26. Salasznyk RM, Klees RF, Boskey A, Plopper GE. Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin‐5. J Cell Biochem 2007; 100:499-514.
27. Saburi E, Atabati H, Kabiri L, Behdari A, Azizi M, Ardeshirylajimi A, et al. Bone morphogenetic protein-7 incorporated polycaprolactone scaffold has a great potential to improve survival and proliferation rate of the human embryonic kidney cells. J Cell Biochem 2019; 120:9859-9868.
28. Tahmasebi A, Enderami SE, Saburi E, Islami M, Yaslianifard S, Mahabadi JA, et al. Micro-RNA-incorporated electrospun nanofibers improve osteogenic differentiation of human-induced pluripotent stem cells. J Biomed Mater Res A 2020; 108:377-386.
 
Iranian Journal of Basic Medical Sciences
Volume 27, Issue 2
February 2024
Pages 188-194

Files

Share

How to cite

Statistics