The effect of eight weeks endurance training and high-fat diet on appetite-regulating hormones in rat plasma

Document Type: Original Article


1 Department of Human Science, Semnan University, Semnan, Iran

2 Exercise Physiology Research Canter, Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Department of Exercise physiology, Faculty of Physical Education and Sports Science, University of Tehran, Tehran, Iran

4 Department of Biostatistics, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

5 Sport Science and Physical Education Research Center, Mashhad, Iran

6 Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Objective(s):Consumption of high-fat foods is one of the major causes of obesity. Physical exercise is a strategy used to counteract obesity. The aim of this study was to investigate the effect of eight weeks endurance training and high-fat diet (HFD) on appetite-regulating hormones in rat plasma.
Materials and Methods:Twenty eight male Wistar rats were randomly divided into four groups: Control group with standard diet (CSD), endurance training with a standard diet (ESD), control group with high-fat diet (CHFD) and endurance training with high-fat diet (EHFD). Twenty-four hr after the last training session, the blood samples were obtained and analyzed for hormones levels.
Results: The significant increased weight gain and food intake and decreased plasma nesfatin-1 and PYY3-36 levels were observed in CHFD group, while exercise under the HFD antagonized these effects. There were no significant changes in ghrelin, insulin and leptin levels in different groups.
Conclusion: These results suggest that exercise can prevent fattening effect of HFD. Probably, performing exercise makes a reduction of food intake and weight gain in rat via the increase in nesfatin-1 and PYY levels. However, further studies are necessary to understand the exact mechanisms involved in this field.


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