The effect of BSO-induced oxidative stress on histologic feature of testis: testosterone secretion and semen parameters in mice

Document Type : Original Article

Authors

1 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran

2 Neuroscience Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Iran

3 Biochemical Department, Faculty of Medicine, Medical Sciences University of Tabriz, Iran

4 Department of Gynecology, Faculty of Medicine, Tabriz university of Medical Sciences, Iran

Abstract

Objective(s): Buthionine sulfoximine (BSO) inhibits synthesis of glutathione as the main intracellular antioxidant. The aim of the present study is to investigate the effect of BSO-induced oxidative stress on histological structure of testis, testosterone secretion and semen parameters.
Materials and Methods: Thirty male BALB/c mice were divided into 3 groups. In control group, the mice did not receive any chemical. In the experimental group, the mice received 2 mmol/kg BSO for 35 days. In the sham group, the mice received the solvent of BSO (0.9% saline). After the treatment, the mice were sacrificed. Their testes were fixed in Buein’s fixative, embedded in paraffin and prepared for histological studies. To assess semen parameters, the sperms were collected from cauda epididymis. Blood samples were used for determination of super oxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH), catalase (CAT) and the serum testosterone level. The data analyzed using ANOVA and Dunnett's tests and SPSS software, version11.5. P- values at 0.05 level considered significant.
Results: Data showed that in experimental group in comparison to control group; the concentration of CAT, GPX, SOD,GSH and the total level of testosterone is reduced while  MDA level is increased  significantly. The number of sperms with progressive motility were decreased               (P<0.001) but sperms with abnormal morphology were increased (P<0.001). Histological studies revealed that the values for tubal differentiation index and spermatogenic index in experimental group were reduced (P<0.001).
Conclusion: It is concluded that exposure to oxidative stress induced by BSO could affect testicular structure and semen parameters.

Keywords

References

1. de Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 1995; 10:15-21.
2. Aitken RJ. Molecular mechanisms regulating human sperm function. Mol Hum Reprod 1997; 3:169-173.
3. Fujii J, Iuchi Y, Matsuki S, Ishii T. Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian J Androl 2003; 5:231-242.
4. Sikka SC. Relative impact of oxidative stress on male reproductive function. Curr Med Chem 2001; 8:851-862.
5. Agarwal A, Prabakaran SA. Mechanism, measurement and prevention of oxidative stress in male reproductive physiology. Indian J Exp Biol 2005; 43:963-974.
6. Rodriguez H, Cutler RG. Critical reviews of oxidative stress and aging. Advances in Basic Science, Diagnostics and Interventions. Singapore: World Scientific Pub Co Inc. 2002; 170-181.
7. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8:616-627.
8. Kodama H, Kuribayashi Y, Gagnon C. Effect of sperm lipid peroxidation on fertilization. J Androl 1996; 17:151-157.
9. Agarwal A, Prabakaran SA. Mechanism, measure-ment, and prevention of oxidative stress in male reproductive physiology. Indian J Exp Biol 2005; 43;963-974.
10.  Garrido N, Meseguer M, Simon C, Pellicer A, Remohi J. Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J Androl 2004; 6:59-65.
11.  Alvarez JG, Sharma RK, Ollero M, Saleh RA, Lopez MC, Thomas AJ Jr, et aI. Increased DNA damage in sperm from leukocytospermics semen samples as determined by the sperm chromatin structure assay. Fertil Steril 2002; 78:319-329.
12.  Shekarriz M, Sharma RK, Thomas AJ Jr, Agarwal A. Positive myeloperoxidase staining (Endtz test) as an indicator of excessive reactive oxygen species formation in semen. J Assist Reprod Genet 1995; 12:70-74.
13. Gomez E, Buckingham DW, Brindle J, Lanzafame F, Irvine DS, Aitken RJ. Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: Correlation with biochemical markers of the cytoplasmic space, oxidative stress and sperm function. J Androl 1996; 17:276-287.
14.  Darley-Usmar V, Wiseman H, Halliwell B. Nitric oxide and oxygen radicals: a question of balance. FEBS Lett 1995; 369:131-135.
15. Ford WC, Whittington K. Antioxidant treatment for male subfertility: a promise that remains unfulfilled. Hum Reprod 1998; 13:1416-1419.
16. Tarin JJ, Brines J, Canno A. Antioxidants may protect against infertility. Hum Reprod 1998; 13:1415-1416.
17.  Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl 2005; 26:349-353.
18. Hughes CM, Lewis SE, McKelvey-Martin VJ, Thompson W. The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum Reprod 1998; 13:1240-1247.
19. Irvin S. Glutathione as a treatment for male infertility. Rev Reprod. 1996; 1:6-12.
20.Chaudher AR, Das P, Singh R. Study of oxidative stress and reduced glutathione levels in seminal plasma of human subjects with different fertility potential. Biomed Res 2008; 19:207-210.
21. Friesen C, Kiess Y, Debatin KM. A critical role of glutathione in determining apoptosis sensitivity and resistance in leukemia cells. Cell Death Differ 2004; 11:573-585.
22. Wroblewski N, Schill WB, Henkel R. Metal chelators change the human sperm motility pattern. Fertil Steril 2003; 79:1584-1589.
23. Niakani a, Farrokhi F, Hasanzadeh S. Decapeptyl ameliorates cyclophosphamide-induced reproductive toxicity in male BALB/c mice: histomorphometric, steriologic and hormonal evidences. Iran J Reprod Med 2013; 11:791-800.
24. Porter KL, Shetty G, Meistrich ML. Testicular edema is associated with spermatogonial arrest in irradiated rats. Endocrinology 2006; 147:1297-1305. 
25. Tash JS, Attardi B, Hild SA, Chakrasali R, Jakkaraj SR, Georg GI. A Novel Potent Indazole Carboxylic Acid Derivative Blocks Spermatogenesis and Is Contraceptive in Rats after a Single Oral Dose. Biol Reprod 2008; 78:1127-1138.
26. Griffith OW. Examination of glutathione and glutathione disulfide using glutathione reductaseand 2- vinylpyridine. Anal Biochem 1980; 106:207-212.
27. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 1969; 27:502-522.
28. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186:407-421.
29. Fairbanks V, Klee GG. Biochemical aspects of haematology. In: Burtis CA, Ashwood ER. ads. Tietze textbook of clinical chemistry. Philadelphia: WB Saunders. 1994; 2020-2021.
30. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70:158–169.
31. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121-126.
32. Ortega AL, Mena S, Estrela JM. Glutathione in cancer cells death. Cancer 2011; 3:1285-1310.
33. Davis KJ. Oxidative stress, antioxidant defenses and damage removal, repair and replacement systems. IUBMB Life 2000; 50:279-289.
34. Emokpae AM, Ojiefo UP, Aisha KG. Antioxidant enzymes and acute phase proteins correlate with marker of lipid peroxide in adult nigerian sickle cell disease patients. Iran J Basic Med Sci 2010; 13:177-182.
35. Ramezani A, Goudarzi I, Lashkarboluki T, Ghorbanian MT, Abrari K, Elahdadi Salmani M. Role of oxidative stress in ethanol-induced neurotoxicity in the developing cerebellum. Iran J Basic Med Sci 2012; 15:965-974.
36. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79:829–43.
37. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril 2000; 73:459–464.
38. Anderson ME. Glutathione: an overview of biosynthesis and modulation. Chem Biol Interact 1998; 111:1-14.
39. Nudell DM, Monoski MM, Hipshultz LI. Common medications and drugs: how they affect male fertility. Urol Clin North Am 2002; 29: 965-973.
40. Fingerova H, Oborna I, Novotny J, Svobodova M, Brezinova J, Radova L. The measurement of reactive oxygen species in human neat semen and in suspended spermatozoa. A comparison. Reprod Biol Endocrinol 2009; 7:118. 
41. Amorim EA, Torres CA, Graham JK, Amorim LS, Santos LV. The hypoosmotic swelling test in fresh rabbit spermatozoa. Anim Reprod Sci 2009; 111:338-343.
42. Sharma RK, Pasqualotto FF, Nelson DR, Thomas AJ Jr, Agarwal A. The reactive oxygen species–total antioxidant capacity score is a new measure of oxidative stress to predict male infertility. Hum Reprod 1999; 14:2801–2807.
43. Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ Jr, et al. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 2004; 81:349–354.
44. Zunjarrao GB, Kavita MM, Jayshree GN, Vandana ZB, Birendra KY. Human seminal oxidative stress: correlation with antioxidants and sperm quality parameters. Ann Biol Res 2011; 2:351-359.
45. Zubkova EV, Robaire B. Effect of glutathione
depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown norway rat. Biol Reprod. 2004; 71:1002-1008. 
46. Maher P. The effects of stress and aging on glutathione metabolism. Ageing Res Rev 2005; 4:288-314.
47. Bhardwaj A, Verma A, Majumdar S, Khanduja KL. Status of vitamin E and reduced glutathione in semen of Oligozoospermic and azoospermic patients. Asian J Androl 2000; 2:225-228.
Iranian Journal of Basic Medical Sciences
Volume 17, Issue 8 - Serial Number 8
August 2014
Pages 606-612

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