The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells

Authors

1 Department of Biology & Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

2 Developmental Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

3 Department of Medical Genetics & Medical Genetic Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Biology, Research & Science Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed   to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage.
Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value Results: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis.
Conclusion: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium

Keywords


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