Association of ADAM33 gene polymorphisms with allergic asthma

Document Type: Original Article

Authors

1 Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

3 Bushehr University of Medical Science, School of Medicine. Allergy and clinical Immunology. Department, Bushehr, Iran

4 Department of Pathology, Omid Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s): Asthma results from the interaction between genetic and environmental factors. ADAM33 gene on chromosome 20p13 is associated with asthma and airway hyperresponsiveness.
Materials and Methods: This is a case-control study, where four SNPs S1 (rs3918396), T1 (rs2280091), T2 (rs2280090), V4 (rs2787094) of ADAM33 gene have been assessed in patients with allergic asthma and normal controls (95 patients and 86 normal). Blood samples of these participants have been genotyped by PCR and the RFLP method.
Results: There was no association between asthmatic patients and polymorphisms of alleles, genotypes and haplotypes of the ADAM33 gene. When categorizing the asthmatic patients in severe, moderate and mild groups, associations in the subcategories of asthmatic patients were found. There were associations between polymorphisms of C allele of T1 SNP with severe asthmatic patients and G allele of V4 SNP with moderate asthmatics respectively (P=0.006, P=0.01). There was a significant association between sensitivity to mite and polymorphism of C allele of T1 SNP (P=0.02). Besides, there was a significant association between sensitivity to weeds and genotype GG of V4 SNP (P=0.05).
Conclusion: Polymorphisms of ADAM33 gene might be associated with severe asthma and sensitivity to aeroallergens in northeast of Iran, but further studies are needed to determine the polymorphisms in this area and other regions of our country.

Keywords


1. Sharma N, Tripathi P, Awasthi S. Role of ADAM33 gene and associated single nucleotide polymorphisms in asthma. Allergy Rhinol ;2011;2:63-70.

2. Entezari A, Mehrabi Y, Varesvazirian M, Pourpak Z, Moin M. A systematic review of recent asthma symptom surveys in Iranian children. Chron Respir Dis 2009; 6:109-114.

3. Al-Khayyat AI, Al-Anazi M, Warsy A, Vazquez-Tello A, Alamri AM, Halwani R, et al. T1 and T2 ADAM33 single nucleotide polymorphisms and the risk of childhood asthma in a Saudi Arabian population: a pilot study. Ann Saudi Med;2012; 32:479-486.

4. Jongepier H, Boezen HM, Dijkstra A, Howard TD, Vonk JM, Koppelman GH, et al. Polymorphisms of the ADAM33 gene are associated with accelerated lung function decline in asthma. Clin Exp Allergy 2004; 34:757-760.

5. Vercelli D. Discovering susceptibility genes for asthma and allergy. Nat Rev Immunol 2008; 8:169-182.

6. Haitchi HM, Powell RM, Shaw TJ, Howarth PH, Wilson SJ, Wilson DI, et al. ADAM33 expression in asthmatic airways and human embryonic lungs. Am J Respir Crit Care Med 2005 ; 171:958-965.

7. Kedda MA, Duffy DL, Bradley B, O'Hehir RE, Thompson PJ. ADAM33 haplotypes are associated with asthma in a large Australian population. Eur J Hum Genet 2006; 14:1027-1036.

8. Schedel M, Depner M, Schoen C, Weiland SK, Vogelberg C, Niggemann B, et al. The role of polymorphisms in ADAM33, a disintegrin and metalloprotease 33, in childhood asthma and lung function in two German populations. Respir Res 2006; 7:91.

9. Tripathi P, Awasthi S, Prasad R, Husain N, Ganesh S. Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population. J Genet; 2011; 90:265-273.

10. Awasthi S, Tripathi P, Ganesh S, Husain N. Association of ADAM33 gene polymorphisms with asthma in Indian children. J Hum Genet; 2011; 56:188-195.

11. Holgate ST, Yang Y, Haitchi HM, Powell RM, Holloway JW, Yoshisue H, et al. The genetics of asthma: ADAM33 as an example of a susceptibility gene. Proc Am Thorac Soc 2006 ; 3:440-443.

12. Van Eerdewegh P, Little RD, Dupuis J, Del Mastro RG, Falls K, Simon J, et al. Association of the ADAM33 gene with asthma and bronchial hyperresponsiveness. Nature 2002 ; 418:426-430.

13. Howard TD, Postma DS, Jongepier H, Moore WC, Koppelman GH, Zheng SL, et al. Association of a disintegrin and metalloprotease 33 (ADAM33) gene with asthma in ethnically diverse populations. J Allergy Clin Immunol 2003; 112:717-722.

14. Elçin Bora ZA-A, Fatih Firinci, Tufan Çankaya, Özlem Giray-Bozkaya, Nevin Uzuner,  Ülgenalp A. ADAM33 Gene Polymorphisms Are Not Associated with Asthma in Turkish Children Pediatric Allergy, Immunology, and Pulmonology. 2012; 25(2): 97-100.

15. Lind DL, Choudhry S, Ung N, Ziv E, Avila PC, Salari K, et al. ADAM33 is not associated with asthma in Puerto Rican or Mexican populations. Am J Respir Crit Care Med 2003; 168:1312-1316.

16. Wang P, Liu QJ, Li JS, Li HC, Wei CH, Guo CH, et al. Lack of association between ADAM33 gene and asthma in a Chinese population. Int J Immunogenet 2006; 33:303-306.

17. Sadeghnejad A, Ohar JA, Zheng SL, Sterling DA, Hawkins GA, Meyers DA, et al. Adam33 polymorphisms are associated with COPD and lung function in long-term tobacco smokers. Respir Res 2009; 10:21.

18. Raby BA, Silverman EK, Kwiatkowski DJ, Lange C, Lazarus R, Weiss ST. ADAM33 polymorphisms and phenotype associations in childhood asthma. J Allergy Clin Immunol 2004 ; 113:1071-1078.

19. Lee JH, Park HS, Park SW, Jang AS, Uh ST, Rhim T, et al. ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics. Clin Exp Allergy 2004 ; 34:860-865.

20. Blakey J, Halapi E, Bjornsdottir US, Wheatley A, Kristinsson S, Upmanyu R, et al. Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax 2005; 60:274-276.

21. Li X, Zhang Y, Zhang J, Xiao Y, Huang J, Tian C, et al. Asthma susceptible genes in Chinese population: a meta-analysis. Respir Res; 2010;11:129.

22. Werner M, Herbon N, Gohlke H, Altmuller J, Knapp M, Heinrich J, et al. Asthma is associated with single-nucleotide polymorphisms in ADAM33. Clin Exp Allergy 2004; 34:26-31.

23. Simpson A, Maniatis N, Jury F, Cakebread JA, Lowe LA, Holgate ST, et al. Polymorphisms in a disintegrin and metalloprotease 33 (ADAM33) predict impaired early-life lung function. Am J Respir Crit Care Med 2005; 172:55-60.

24. van Diemen CC, Postma DS, Vonk JM, Bruinenberg M, Schouten JP, Boezen HM. A disintegrin and metalloprotease 33 polymorphisms and lung function decline in the general population. Am J Respir Crit Care Med 2005; 172:329-333.

25. Foley SC, Mogas AK, Olivenstein R, Fiset PO, Chakir J, Bourbeau J, et al. Increased expression of ADAM33 and ADAM8 with disease progression in asthma. J Allergy Clin Immunol 2007; 119:863-871.

26. Hirota T, Hasegawa K, Obara K, Matsuda A, Akahoshi M, Nakashima K, et al. Association between ADAM33 polymorphisms and adult asthma in the Japanese population. Clin Exp Allergy 2006; 36:884-891.

27. Thongngarm T, Jameekornrak A, Limwongse C, Sangasapaviliya A, Jirapongsananuruk O, Assawamakin A, et al. Association between ADAM33 polymorphisms and asthma in a Thai population. Asian Pac J Allergy Immunol 2008; 26:205-211.

28. Bijanzadeh M, Ramachandra NB, Mahesh PA, Mysore RS, Kumar P, Manjunath BS, et al. Association of IL-4 and ADAM33 gene polymorphisms with asthma in an Indian population. Lung; 2010; 188:415-422.

29. El-Falaki M WM, Ezzat G, Mokhtar D, El-Baz M,       Hamed D. A disintegrin and metalloproteinase 33 (ADAM33) gene polymorphism association with asthma in Egyptian children. Egyp J Med Hum Genet 2013; 14:55-62.

30. Vergara CI, Acevedo N, Jimenez S, Martinez B, Mercado D, Gusmao L, et al. A Six-SNP haplotype of ADAM33 is associated with asthma in a population of Cartagena, Colombia. Int Arch Allergy Immunol 152:32-40.