Association between HLA-DQB1 alleles and HAM/TSP patients in Khorasan Province

Document Type: Original Article

Authors

1 Immunology Research Group, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

2 Neurology Department and HTLVI Foundation, Ghaem Medical Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Genetics Department School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Objective(s): HTLVI-1 is the first human retrovirus with limited endemic regions in the world. The epidemiological studies have shown that the genetic background and immune response to the virus have a significant role in HTLV-I-associated diseases.  Among the genes are involved in HTLV-I infection, the role of human leukocytes antigen (HLA) have been studied in different population. In the present study we examined the association between HLA-DQB1 alleles and HTLV-I infection in HAM/TSP patients, HTLV-I carriers and healthy controls in north east of Iran, Mashhad.
Materials and Methods:The blood samples of 16 patients with HAM/TSP, 20 HTLV-1 carriers, and 30 healthy individuals were taken and DNA was extracted by salting out method.  HLA-DQB1 typing was performed using PCR-SSP method and the frequency of HLA-DQB1 alleles were compared by Fischer Exact Test.
Results:There was a significant difference between HAM/TSP patients and healthy controls in the frequency of HLA-DQB1*07 (P=0.004, RR=7). Furthermore, we found that possession of HLA- DQB1*02 or HLA-DQB1*05 increased the risk of disease 1.5 times.
Conclusion: The data presented here suggest that both HLA-DQB1*07 and HLA-DQB1*06 are associated with disease development.

Keywords


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