Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine

Authors

1 Health Management Department, Quality Assurance Deputy, Razi Vaccine & Serum Research Institute, Karaj, Iran

2 Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

3 Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

4 Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran

5 Anatomy Department, School of Medicine, Tarbiat Modares University, Tehran, Iran

6 Omid Institute for Advance Biomodels, Incubation Center for Medical Devices, Tehran University of Medical Sciences, Tehran, Iran

7 Virology Department, Pasteur Institute of Iran, Tehran, Iran

Abstract

Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs.
Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] .
Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods.
Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs.

Keywords


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