Plasma Nitric Oxide and Acute Phase Proteins after Moderate and Prolonged Exercises

Document Type: Original Article


1 Department of Chemical Pathology and Immunology, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ago-iwoye, Nigeria

2 Department of Chemical Pathology and Immunology, University College Hospital, Ibadan, Nigeria


The aim of this study was to evaluate plasma levels of nitric oxide (NO) and certain acute phase proteins
(caeruloplasmin, transferrin, haptoglobin, C-reactive protein) in Nigerian subjects after short and prolonged exercises.
Materials and Methods
A total of 57 subjects (34 males and 23 females) between ages of 19 and 45 years participated in this study and were
divided into three groups: Group 1 (Prolonged exercise) consisted of footballers (10 males and 9 females) who played
football for at least two hrs daily; Group II (Moderate exercise) consisted of individuals (14 males and 5 females) who
played football for 30 min 3 times a week; Group III (10 males and 9 females) were sedentary workers, who rarely had
any form of physical exercise. The plasma samples were assayed for NO, C-reactive protein (CRP), caeruloplasmin,
haptoglobin, and transferrin using spectrophotometer and immunoplates. Statistical analysis was done using the
The result showed that there was a significant reduction in the level of NO in prolonged exercise (P<0.05) when
compared with control subjects while the increase of NO in subjects with moderate exercise was not statistically
significant when compared with control subjects. C-reactive protein was significantly increased (P< 0.01) while
transferrin and haptoglobin were significantly reduced (P< 0.001 and P< 0.01 respectively) in subjects with prolonged
exercise when compared with control subjects. In moderate exercise, haptoglobin was significantly reduced (P< 0.05)
while the reduction in the levels of caeruloplasmin and transferrin was not statistically significant when compared with
control subjects. The mean level of CRP was significantly raised in prolonged exercise compared with controls or
moderate exercise while the level of caeruloplasmin was significantly reduced in prolonged exercise compared with
controls or moderate exercise.
Moderate exercises should be encouraged.


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